| Literature DB >> 28853502 |
Wang Ying1, Wang Qimin2, Li Jinghua3, Han Jinhong4, Wang Lili3, Chao Chen5, Zhou Jianhua2, Tong Lei2, Lu Xufei6, Zhou Yuan1, Liao Yixiang2, He Zongxuan2, Li Ning7, Cao Lei7, Liu Wenjun8, Chen Zhenggang9.
Abstract
Objective This study aims to investigate the effect of geranylgeranyltransferaseⅠ (GGTase-Ⅰ) on the proliferation and growth of tongue squamous cancer cells. Methods Three small interfering RNAs (siRNAs) were designed on the basis of the GGTase-Ⅰ sequence in GeneBank. These siRNAs were then transfected into tongue squamous cancer cells Cal-27. The mRNA and protein expression of GGTase-Ⅰ and RhoA were examined by real-time quantitative polymerase chain reaction and Western blotting, respectively. The expression of Cyclin D1 and p21 were examined by Western blotting. The proliferation and growth ability were analyzed by cell counting kit-8 assay and flow cytometry. Results The mRNA and protein expression of GGTase-Ⅰ in Cal-27 was reduced significantly after the GGTase-Ⅰ siRNAs were transfected (P<0.05). No significant difference in RhoA mRNA and protein expression was detected (P>0.05). Cyclin D1 expression decreased, whereas p21 expression increased significantly. The cell cycle was altered, and the growth-proliferative activity was inhibited (P<0.05). Conclusion GGTase-Ⅰ siRNA can inhibit the expression of GGTase-Ⅰ and the proliferative activity of tongue squamous cancer cells. GGTase-Ⅰ may be a potential target for gene therapy in tongue squamous cell cancer.Entities:
Keywords: Cyclin D1; RhoA; geranylgeranyltransferaseⅠ; p21; proliferation; tongue squamous cancer
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Year: 2017 PMID： 28853502 PMCID： PMC7030231 DOI： 10.7518/hxkq.2017.04.006
Source DB: PubMed Journal: Hua Xi Kou Qiang Yi Xue Za Zhi ISSN： 1000-1182