Electrochemiluminescent resonance energy transfer of polymer dots for aptasensing.

This work designed a three-component polymer for the preparation of polymer dots (Pdots). The polymer contained 9-(diphenylmethylene)-9H-fluorene (DPF), 9,9-dioctyl-9H-fluorene (DOF) and 1,1'-binaphthyl moieties, and was synthesized via Pd-catalyzed Suzuki reaction. It exhibited obvious yellow-colored aggregation-induced emission (AIE) for fluorescence enhancement at 543nm via an intramolecular fluorescence resonance energy transfer from DOF moiety to DPF moiety. The Pdots prepared by nanoprecipitation could be conveniently cast on electrode surface and showed a stable anodic electrochemiluminescence (ECL) emission in the presence of triethylamine as a co-reactant. The ECL emission could be effectively quenched by rhodamine B via resonance energy transfer, which led to an "off-on" switch for the design of ECL sensing methodology. Using Pb2+ as a target model, an ECL aptasensor for the detection of trace Pb2+ was proposed, which showed a linear range of 100pM to 1.0μM with a detection limit down to 38.0pM This work demonstrated the first Pdots prepared with AIE-active polymer for highly efficient ECL sensing.