Maria Byrne1,2, Demian Koop1, Valerie B Morris2, Juanita Chui1, Gregory A Wray3, Paula Cisternas1. 1. School of Medical Sciences, The University of Sydney, NSW, Australia. 2. School of Life and Environmental Sciences, The University of Sydney, NSW, Australia. 3. Department of Biology and Center for Genomic and Computational Biology, Duke University, Durham, North Carolina.
Abstract
BACKGROUND: Photoreception-associated genes of the Pax-Six-Eya-Dach network (PSEDN) are deployed for many roles in addition to photoreception development. In this first study of PSEDN genes during development of the pentameral body in sea urchins, we investigated their spatial expression in Heliocidaris erythrogramma. RESULTS: Expression of PSEDN genes in the hydrocoele of early (Dach, Eya, Six1/2) and/or late (Pax6, Six3/6) larvae, and the five hydrocoele lobes, the first morphological expression of pentamery, supports a role in body plan development. Pax6, Six1/2, and Six3/6 were localized to the primary and/or secondary podia and putative sensory/neuronal cells. Six1/2 and Six3/6 were expressed in the neuropil region in the terminal disc of the podia. Dach was localized to spines. Sequential up-regulation of gene expression as new podia and spines formed was evident. Rhabdomeric opsin and pax6 protein were localized to cells in the primary podia and spines. CONCLUSIONS: Our results support roles for PSEDN genes in development of the pentameral body plan, contributing to our understanding of how the most unusual body plan in the Bilateria may have evolved. Development of sensory cells within the Pax-Six expression field is consistent with the role of these genes in sensory cell development in diverse species. Developmental Dynamics 247:239-249, 2018.
BACKGROUND: Photoreception-associated genes of the Pax-Six-Eya-Dach network (PSEDN) are deployed for many roles in addition to photoreception development. In this first study of PSEDN genes during development of the pentameral body in sea urchins, we investigated their spatial expression in Heliocidaris erythrogramma. RESULTS: Expression of PSEDN genes in the hydrocoele of early (Dach, Eya, Six1/2) and/or late (Pax6, Six3/6) larvae, and the five hydrocoele lobes, the first morphological expression of pentamery, supports a role in body plan development. Pax6, Six1/2, and Six3/6 were localized to the primary and/or secondary podia and putative sensory/neuronal cells. Six1/2 and Six3/6 were expressed in the neuropil region in the terminal disc of the podia. Dach was localized to spines. Sequential up-regulation of gene expression as new podia and spines formed was evident. Rhabdomeric opsin and pax6 protein were localized to cells in the primary podia and spines. CONCLUSIONS: Our results support roles for PSEDN genes in development of the pentameral body plan, contributing to our understanding of how the most unusual body plan in the Bilateria may have evolved. Development of sensory cells within the Pax-Six expression field is consistent with the role of these genes in sensory cell development in diverse species. Developmental Dynamics 247:239-249, 2018.
Authors: Gregory A Cary; Brenna S McCauley; Olga Zueva; Joseph Pattinato; William Longabaugh; Veronica F Hinman Journal: Nat Commun Date: 2020-12-04 Impact factor: 14.919
Authors: Guilherme Gainett; Jesús A Ballesteros; Charlotte R Kanzler; Jakob T Zehms; John M Zern; Shlomi Aharon; Efrat Gavish-Regev; Prashant P Sharma Journal: BMC Genomics Date: 2020-11-23 Impact factor: 3.969
Authors: Maria Byrne; Demian Koop; Dario Strbenac; Paula Cisternas; Regina Balogh; Jean Yee Hwa Yang; Phillip L Davidson; Gregory Wray Journal: DNA Res Date: 2020-02-01 Impact factor: 4.458