Weiwei Liang1, Qunhui Yang1, Gaofeng Wu1, Shumei Lin1, Jiancheng Yang1, Ying Feng1, Jianmin Hu2. 1. Liaoning Provincial Key Laboratory of Zoonoses, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, 110866, People's Republic of China. 2. Liaoning Provincial Key Laboratory of Zoonoses, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, Liaoning, 110866, People's Republic of China. hujianmin59@163.com.
Abstract
OBJECTIVE: In order to find the effects and mechanism of taurine and L-arginine on the apoptosis of VSMCs in insulin resistance hypertensive rats. METHODS: 25% fructose were administered in the drinking water to Wistar rats for 12 weeks to induce the insulin resistance hypertensive model. Apoptosis of VSMCs was identified by TUNEL. The expressions of Bax protein and Bcl-2 protein were examined by immunohistochemistry. RESULTS: High level of fructose significantly suppressed the apoptosis of VSMCs. Taurine and L-arginine promoted apoptosis of VSMCs via increasing the Bax protein expression and decreasing the Bcl-2 protein expression. CONCLUSIONS: Taurine and L-arginine have obvious anti-hypertensive effects in insulin resistance hypertensive rats, and its mechanism might partially be associated with attenuating vascular remodeling by promoting apoptosis in VSMCs. In addition, the combined medication of taurine and L-arginine was more effective than single medication.
OBJECTIVE: In order to find the effects and mechanism of taurine and L-arginine on the apoptosis of VSMCs in insulin resistance hypertensiverats. METHODS: 25% fructose were administered in the drinking water to Wistar rats for 12 weeks to induce the insulin resistance hypertensive model. Apoptosis of VSMCs was identified by TUNEL. The expressions of Bax protein and Bcl-2 protein were examined by immunohistochemistry. RESULTS: High level of fructose significantly suppressed the apoptosis of VSMCs. Taurine and L-arginine promoted apoptosis of VSMCs via increasing the Bax protein expression and decreasing the Bcl-2 protein expression. CONCLUSIONS:Taurine and L-arginine have obvious anti-hypertensive effects in insulin resistance hypertensiverats, and its mechanism might partially be associated with attenuating vascular remodeling by promoting apoptosis in VSMCs. In addition, the combined medication of taurine and L-arginine was more effective than single medication.