Literature DB >> 28848270

Molecular identification of Mecistocirrus digitatus and Toxocara vitulorum in the mithun (Bos frontalis) from north-east India.

Jayanta Kumar Chamuah1, O K Raina2, A Sakhrie1, N Gama3.   

Abstract

Mecistocirrus digitatus and Toxocara vitulorum are common pathogenic nematode parasites of mithun (Bos frontalis). Species identification by morphological features was confirmed by molecular identification of M. digitatus and T. vitulorum. The internal transcribed spacer-2 (ITS-2) region and beta tubulin gene of M. digitatus were polymerase chain reaction (PCR) amplified and sequenced. ITS-2 sequence analysis showed 100% homology with other isolates of M. digitatus and 83% identity with Haemonchus contortus and H. placei, respectively. Likewise, ITS-1 and ITS-2 sequences of T. vitulorum were PCR amplified and sequenced. Sequence analysis of these internal transcribed spacers from five worms of the parasite from mithun showed no intraspecific variations with T. vitulorum isolates from domestic ruminants.

Entities:  

Keywords:  Beta tubulin gene; Internal transcribed spacers; Mithun; Parasites

Year:  2017        PMID： 28848270      PMCID： PMC5555924          DOI： 10.1007/s12639-017-0879-5

Source DB:  PubMed          Journal:  J Parasit Dis        ISSN： 0971-7196


  7 in total

1.  Toxocara vitulorum in a bison (Bison bison) herd from western Canada.

Authors:  Murray R Woodbury; Shelagh Copeland; Brent Wagner; Champika Fernando; Janet E Hill; Cathy Clemence
Journal:  Can Vet J       Date:  2012-07       Impact factor: 1.008

2.  A molecular tool for species identification and benzimidazole resistance diagnosis in larval communities of small ruminant parasites.

Authors:  A Silvestre; J F Humbert
Journal:  Exp Parasitol       Date:  2000-08       Impact factor: 2.011

3.  Economic analysis of risk of gastrointestinal parasitic infection in cattle in North Eastern States of India.

Authors:  S Bandyopadhyay; S Mandal; K K Datta; P Devi; S De; A K Bera; D Bhattacharya
Journal:  Trop Anim Health Prod       Date:  2010-04-22       Impact factor: 1.559

4.  Toxocara canis and Toxocara vitulorum: molecular characterization, discrimination, and phylogenetic analysis based on mitochondrial (ATP synthase subunit 6 and 12S) and nuclear ribosomal (ITS-2 and 28S) genes.

Authors:  Susiji Wickramasinghe; Lalani Yatawara; R P V J Rajapakse; Takeshi Agatsuma
Journal:  Parasitol Res       Date:  2009-02-17       Impact factor: 2.289

5.  Toxocara vitulorum in American bison (Bison bison) calves.

Authors:  E Goossens; P Dorny; H Vervaecke; C Roden; F Vercammen; J Vercruysse
Journal:  Vet Rec       Date:  2007-04-21       Impact factor: 2.695

6.  Molecular characterization of veterinary important trematode and cestode species in the mithun Bos frontalis from north-east India.

Authors:  J K Chamuah; O K Raina; H Lalrinkima; S S Jacob; M Sankar; A Sakhrie; S Lama; P S Banerjee
Journal:  J Helminthol       Date:  2015-09-07       Impact factor: 2.170

7.  Differentiation of Haemonchus placei from H. contortus (Nematoda: Trichostrongylidae) by the ribosomal DNA second internal transcribed spacer.

Authors:  L A Stevenson; N B Chilton; R B Gasser
Journal:  Int J Parasitol       Date:  1995-04       Impact factor: 3.981
  7 in total

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