Literature DB >> 28844721

A multiplex real-time PCR for the direct, fast, economic and simultaneous detection of the carbapenemase genes blaKPC, blaNDM, blaVIM and blaOXA-48.

Daniel Weiß1, Ines Engelmann2, Sascha D Braun2, Stefan Monecke3, Ralf Ehricht2.   

Abstract

A novel multiplex real-time PCR was designed to detect the clinically most important carbapenemase genes, blaKPC, blaVIM, blaNDM and blaOXA-48. The multiplex assay was verified testing genomic DNA of 24 carbapenemase-producing strains. It was validated using a blinded panel of 82 carbapenemase-producing and 50 non-producing isolates by direct colony PCR.
Copyright © 2017 Elsevier B.V. All rights reserved.

Keywords:  CPO; Carbapenemases; Colony PCR; Hospital; Microarray; Multiplex; Real-time PCR

Mesh：

Substances：

Year:  2017        PMID： 28844721     DOI： 10.1016/j.mimet.2017.08.017

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  8 in total

Review 1.  Carbapenemase-producing Enterobacteriaceae in animals and methodologies for their detection.

Authors:  Rebecca E V Anderson; Patrick Boerlin
Journal:  Can J Vet Res       Date:  2020-01       Impact factor: 1.310

2.  Detection of carbapenemases blaOXA48-blaKPC-blaNDM-blaVIM and extended-spectrum-β-lactamase blaOXA1-blaSHV-blaTEM genes in Gram-negative bacterial isolates from ICU burns patients.

Authors:  Muhammad Hayat Haider; Timothy D McHugh; Kerry Roulston; Liã Bárbara Arruda; Zahra Sadouki; Saba Riaz
Journal:  Ann Clin Microbiol Antimicrob       Date:  2022-05-19       Impact factor: 6.781

3.  Bacterial colonization dynamics and antibiotic resistance gene dissemination in the hospital environment after first patient occupancy: a longitudinal metagenetic study.

Authors:  Tilman E Klassert; Rasmus Leistner; Cristina Zubiria-Barrera; Magdalena Stock; Mercedes López; Robert Neubert; Dominik Driesch; Petra Gastmeier; Hortense Slevogt
Journal:  Microbiome       Date:  2021-08-11       Impact factor: 14.650

4.  First Report of bla CTX-M-167, bla SHV-1, and bla TEM-1B Carrying Klebsiella pneumonia Showing High-Level Resistance to Carbapenems.

Authors:  Shirong Li; Siquan Shen; Li Ding; Renru Han; Yan Guo; Dandan Yin; Ming Guan; Fupin Hu
Journal:  Front Microbiol       Date:  2022-07-07       Impact factor: 6.064

5.  Occurrence, Phenotypic and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Healthy Turkeys in Northern Egypt.

Authors:  Amira A Moawad; Helmut Hotzel; Hafez M Hafez; Hazem Ramadan; Herbert Tomaso; Sascha D Braun; Ralf Ehricht; Celia Diezel; Dominik Gary; Ines Engelmann; Islam M Zakaria; Reem M Reda; Samah Eid; Momtaz A Shahien; Heinrich Neubauer; Stefan Monecke
Journal:  Antibiotics (Basel)       Date:  2022-08-08

6.  Antimicrobial resistance-Do we share more than companionship with our dogs?

Authors:  Mari Røken; Kristin Forfang; Yngvild Wasteson; Anita Haug Haaland; Hans Geir Eiken; Snorre B Hagen; Ane Mohn Bjelland
Journal:  J Appl Microbiol       Date:  2022-05-29       Impact factor: 4.059

7.  Targeted Molecular Detection of Nosocomial Carbapenemase-Producing Gram-Negative Bacteria-On Near- and Distant-Patient Surfaces.

Authors:  Claudia Stein; Isabel Lange; Jürgen Rödel; Mathias W Pletz; Frank Kipp
Journal:  Microorganisms       Date:  2021-05-31

8.  Occurrence of NDM-1, VIM-1, and OXA-10 Co-Producing Providencia rettgeri Clinical Isolate in China.

Authors:  Siquan Shen; Xiangning Huang; Qingyu Shi; Yan Guo; Yang Yang; Dandan Yin; Xun Zhou; Li Ding; Renru Han; Hua Yu; Fupin Hu
Journal:  Front Cell Infect Microbiol       Date:  2022-01-03       Impact factor: 5.293
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.