Kamilla Felipe do Nascimento1, Flora Martinez Figueira Moreira2, Joyce Alencar Santos3, Candida Aparecida Leite Kassuya4, Julio Henrique Rosa Croda5, Claudia Andrea Lima Cardoso6, Maria do Carmo Vieira7, Ana Lúcia Tasca Góis Ruiz8, Mary Ann Foglio9, João Ernesto de Carvalho10, Anelise Samara Nazari Formagio11. 1. Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados UFGD, MS, Brazil. Electronic address: kamila_felipe@yahoo.com.br. 2. Faculty of Health Sciences, Federal University of Grande Dourados UFGD, MS, Brazil. Electronic address: f.floramoreira@gmail.com. 3. Faculty of Health Sciences, Federal University of Grande Dourados UFGD, MS, Brazil. Electronic address: joycesantos@ufgd.edu.br. 4. Faculty of Health Sciences, Federal University of Grande Dourados UFGD, MS, Brazil. Electronic address: candida2005@gmail.com. 5. Faculty of Health Sciences, Federal University of Grande Dourados UFGD, MS, Brazil; Oswaldo Cruz Foundation, Campo Grande, MS, Brazil. Electronic address: juliocroda@ufgd.edu.br. 6. Chemistry, State University of Mato Grossodo Sul UEMS, MS, Brazil. Electronic address: claudia@uems.br. 7. Faculty of Agricultural Sciences, Federal University of Grande Dourados UFGD, Mato Grosso do Sul State, Brazil. Electronic address: mariavieira@ufgd.edu.br. 8. Multidisciplinary Center for Chemical, Biological, and Agricultural Research, University of Campinas Unicamp, SP, Brazil. Electronic address: analucia@cpqba.unicamp.br. 9. Multidisciplinary Center for Chemical, Biological, and Agricultural Research, University of Campinas Unicamp, SP, Brazil. Electronic address: foglioma@cpqba.unicamp.br. 10. Multidisciplinary Center for Chemical, Biological, and Agricultural Research, University of Campinas Unicamp, SP, Brazil. Electronic address: carvalho_je@yahoo.com.br. 11. Faculty of Biological and Environmental Sciences, Federal University of Grande Dourados UFGD, MS, Brazil; Faculty of Health Sciences, Federal University of Grande Dourados UFGD, MS, Brazil. Electronic address: aneliseformagio@ufgd.edu.br.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Leaves from Psidium guineense Sw. are used in popular medicine for the treatment of inflammatory disease. However, there is no scientific evidence demonstrating this activity. AIM OF THE STUDY: To evaluate the antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities of the essential oil of P. guineense and spathulenol (a major constituent). The study was conducted in part to provide evidence supporting the ethnobotanical use of the leaves of this species. MATERIAL AND METHODS: The essential oil (EOPG) was extracted from the leaves of P. guineense by hydrodistillation and analysed by gas chromatography-mass spectrometry (GC-MS). The major compound, spathulenol (PG-1), was isolated in a chromatographic column and characterized by nuclear magnetic resonance (NMR). EOPG and PG-1 were evaluated in vitro for antioxidant activity by DPPH, ABTS and MDA methods; anti-inflammatory potential was assessed using two models, including pleurisy and oedema, in mice. The impact of EOPG and PG-1 on cell proliferation was determined via spectrophotometric quantification of the cellular protein content using a sulforhodamine B assay, and anti-Mycobacterium tuberculosis activity was determined using the REMA method. RESULTS: A total of 38 components were identified from the EOPG, with the sesquiterpenic alcohol spathulenol (PG-1) (80.7%) being the major constituent. EOPG and PG-1 exhibited the highest antioxidant activities in the DPPH and MDA system compared with reference standard, with IC50 values ranging from 26.13 to 85.60μg/mL. Oral administration of EOPG and PG-1 showed significant inhibition in the Cg-induced mice paw oedema and pleurisy model. The EOPG (GI50 = 0.89μg/mL) and PG-1 (GI50 = 49.30μg/mL) were particularly effective against the ovarian cancer cell line. Both showed moderate antimycobacterial activity. CONCLUSION: For the first time, this study demonstrated the antioxidant, anti-inflammatory, antiproliferative and antimycobacterial properties of the essential oil of P. guineense (leaves were collected in Dourados-MS) and spathulenol, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
ETHNOPHARMACOLOGICAL RELEVANCE: Leaves from Psidium guineense Sw. are used in popular medicine for the treatment of inflammatory disease. However, there is no scientific evidence demonstrating this activity. AIM OF THE STUDY: To evaluate the antioxidant, anti-inflammatory, antiproliferative and antimycobacterial activities of the essential oil of P. guineense and spathulenol (a major constituent). The study was conducted in part to provide evidence supporting the ethnobotanical use of the leaves of this species. MATERIAL AND METHODS: The essential oil (EOPG) was extracted from the leaves of P. guineense by hydrodistillation and analysed by gas chromatography-mass spectrometry (GC-MS). The major compound, spathulenol (PG-1), was isolated in a chromatographic column and characterized by nuclear magnetic resonance (NMR). EOPG and PG-1 were evaluated in vitro for antioxidant activity by DPPH, ABTS and MDA methods; anti-inflammatory potential was assessed using two models, including pleurisy and oedema, in mice. The impact of EOPG and PG-1 on cell proliferation was determined via spectrophotometric quantification of the cellular protein content using a sulforhodamine B assay, and anti-Mycobacterium tuberculosis activity was determined using the REMA method. RESULTS: A total of 38 components were identified from the EOPG, with the sesquiterpenic alcohol spathulenol (PG-1) (80.7%) being the major constituent. EOPG and PG-1 exhibited the highest antioxidant activities in the DPPH and MDA system compared with reference standard, with IC50 values ranging from 26.13 to 85.60μg/mL. Oral administration of EOPG and PG-1 showed significant inhibition in the Cg-induced micepaw oedema and pleurisy model. The EOPG (GI50 = 0.89μg/mL) and PG-1 (GI50 = 49.30μg/mL) were particularly effective against the ovarian cancer cell line. Both showed moderate antimycobacterial activity. CONCLUSION: For the first time, this study demonstrated the antioxidant, anti-inflammatory, antiproliferative and antimycobacterial properties of the essential oil of P. guineense (leaves were collected in Dourados-MS) and spathulenol, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
Authors: Igor A Schepetkin; Gulmira Özek; Temel Özek; Liliya N Kirpotina; Andrei I Khlebnikov; Mark T Quinn Journal: Molecules Date: 2021-06-15 Impact factor: 4.411
Authors: Moldir A Zhandabayeva; Kaldanay K Kozhanova; Assyl K Boshkayeva; Valeriy A Kataev; Gulbaram O Ustenova; Nadezhda G Gemejiyeva; Zhanar A Iskakbayeva Journal: Int J Biomater Date: 2021-07-21
Authors: Pablo Luis B Figueiredo; Renan C Silva; Joyce Kelly R da Silva; Chieno Suemitsu; Rosa Helena V Mourão; José Guilherme S Maia Journal: Chem Cent J Date: 2018-05-10 Impact factor: 4.215