Soumaya Marzouki1, Ines Bini Dhouib2, Chaouki Benabdessalem1, Raja Rekik1, Raoudha Doghri3, Ammar Maroueni4, Zakaria Bellasfar4, Saloua Fazaa5, Jihene Bettaieb6, Mohamed Ridha Barbouche6, Melika Ben Ahmed7. 1. Laboratory of Transmission, Control and Immunobiology of Infections, LR11IPT02, Institut Pasteur de Tunis, Tunis, Tunisia. 2. Laboratory of Venoms and Therapeutic Biomolecules, LR11IPT08, Institut Pasteur de Tunis, Tunis, Tunisia; Laboratory of Endocrinology, Faculté des Sciences de Tunis, Tunis, Tunisia. 3. Laboratory of Anatomo-Pathology, Hôpital Salah Azaiez, Tunis, Tunisia. 4. Animal Unit, Institut Pasteur de Tunis, Tunis, Tunisia. 5. Laboratory of Endocrinology, Faculté des Sciences de Tunis, Tunis, Tunisia. 6. Laboratory of Transmission, Control and Immunobiology of Infections, LR11IPT02, Institut Pasteur de Tunis, Tunis, Tunisia; Faculty of Medicine of Tunis, Tunis El Manar University, Tunis, Tunisia. 7. Laboratory of Transmission, Control and Immunobiology of Infections, LR11IPT02, Institut Pasteur de Tunis, Tunis, Tunisia; Faculty of Medicine of Tunis, Tunis El Manar University, Tunis, Tunisia. Electronic address: melika.benahmed@pasteur.tn.
Abstract
AIMS: Acetamiprid (ACE) is an insecticide of the neonicotinoid family, the most widely used in the world. Herein, we assessed the effect of ACE on either the humoral or cellular immune responses of rodents. We also evaluated the role of curcumin in the restoration of altered immune responses after ACE treatment. METHODS: Five groups of five Swiss Albino mice were immunized intraperitoneally with the recombinant form of CFP32, a virulence factor of Mycobacterium tuberculosis. One group received ACE (5mg/kg) during 61days, a second one received ACE associated with curcumin (100mg/kg). Three control groups were included; one untreated, the second received corn oil and the third received curcumin alone. The humoral immune response was assessed by ELISA testing the anti-rCFP32 antibody concentrations in the serum. The cellular immune response was assessed by analyzing the cellular proliferation of the splenocytes stimulated in vitro by a mitogen or rCFP32. RESULTS: The ACE-treated mice showed a significant immunosuppression of the specific humoral response with a restorative effect of curcumin when administered with ACE. Similarly, ACE significantly decreased the level of splenocyte proliferation after either a non specific or a specific activation. Curcumin partially restores the antigen specific cellular immune response. Moreover, when administered alone, curcumin significantly inhibits the proliferative responses to the mitogen confirming its anti-mitogenic effect. Histological analysis showed alteration of spleens of mice exposed to ACE. SIGNIFICANCE: Altogether, our data indicated that ACE could potentially be harmful to the immune system.
AIMS: Acetamiprid (ACE) is an insecticide of the neonicotinoid family, the most widely used in the world. Herein, we assessed the effect of ACE on either the humoral or cellular immune responses of rodents. We also evaluated the role of curcumin in the restoration of altered immune responses after ACE treatment. METHODS: Five groups of five Swiss Albino mice were immunized intraperitoneally with the recombinant form of CFP32, a virulence factor of Mycobacterium tuberculosis. One group received ACE (5mg/kg) during 61days, a second one received ACE associated with curcumin (100mg/kg). Three control groups were included; one untreated, the second received corn oil and the third received curcumin alone. The humoral immune response was assessed by ELISA testing the anti-rCFP32 antibody concentrations in the serum. The cellular immune response was assessed by analyzing the cellular proliferation of the splenocytes stimulated in vitro by a mitogen or rCFP32. RESULTS: The ACE-treated mice showed a significant immunosuppression of the specific humoral response with a restorative effect of curcumin when administered with ACE. Similarly, ACE significantly decreased the level of splenocyte proliferation after either a non specific or a specific activation. Curcumin partially restores the antigen specific cellular immune response. Moreover, when administered alone, curcumin significantly inhibits the proliferative responses to the mitogen confirming its anti-mitogenic effect. Histological analysis showed alteration of spleens of mice exposed to ACE. SIGNIFICANCE: Altogether, our data indicated that ACE could potentially be harmful to the immune system.
Authors: Antonio Hernandez Jerez; Paulien Adriaanse; Philippe Berny; Tamara Coja; Sabine Duquesne; Andreas Focks; Marina Marinovich; Maurice Millet; Olavi Pelkonen; Silvia Pieper; Aaldrik Tiktak; Christopher Topping; Anneli Widenfalk; Martin Wilks; Gerrit Wolterink; Maj Rundlöf; Alessio Ippolito; Alberto Linguadoca; Laura Martino; Martina Panzarea; Andrea Terron; Annette Aldrich Journal: EFSA J Date: 2022-01-24
Authors: Shimaa M Abou-Zeid; Samira H Aljuaydi; Huda O AbuBakr; Enas A Tahoun; Alessandro Di Cerbo; Mahmoud Alagawany; Samah R Khalil; Mayada R Farag Journal: Mar Drugs Date: 2021-09-18 Impact factor: 5.118