| Literature DB >> 28842904 |
Robert N Judson1,2, Marcela Low3, Christine Eisner3, Fabio M Rossi3.
Abstract
Fibro/Adipogenic Progenitors (FAPs) are a multipotent progenitor population resident in skeletal muscle. During development and regeneration, FAPs provide trophic support to myogenic progenitors that is required for muscle fiber maturation and specification. FAPs also represent a major cellular source of fibrosis in degenerative disease states, highlighting them as a potential cellular target for anti-fibrotic muscle therapies. Effective and reproducible methods to isolate and culture highly purified FAP populations are therefore critical to further understand their biology. Here, we describe a fluorescent activated cell sorting (FACS) based protocol to isolate CD31-/CD45-/Integrin-α7-/Sca1+ FAPs from murine skeletal muscle including details of tissue collection and enzymatic muscle digestion. We also incorporate optimized methods of expanding and differentiated FAPs in vitro. Together, this protocol provides a complete workflow to study skeletal muscle derived FAPs and compliments downstream analytical, drug screening, and disease modeling applications.Entities:
Keywords: FAPs; Fibro-adipogenic progenitors; Fibrosis; MSCs; Mesenchymal progenitors; Myogenesis; Satellite cells; Skeletal muscle; Skeletal muscle regeneration
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Year: 2017 PMID： 28842904 DOI： 10.1007/978-1-4939-7283-8_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745