H Chen1,2, S Ding3,4, M Zhou1, X Wu5, X Liu2, J Liu6, Y Wu2, D Liu5. 1. Southern Medical University, Guangzhou, China. 2. Inner Mongolia People's Hospital, Inner Mongolia, China. 3. Southern Medical University, Guangzhou, China. shifangding1@163.com. 4. Wuhan General Hospital of Guangzhou Military Region, 627 Wuyue Road, 430070, Wuhan, Hubei, China. shifangding1@163.com. 5. Inner Mongolia Medical University, Inner Mongolia, China. 6. Wuhan General Hospital of Guangzhou Military Region, 627 Wuyue Road, 430070, Wuhan, Hubei, China.
Abstract
OBJECTIVE: A decreased plasma high density lipoprotein (HDL) cholesterol level is a strong risk factor for coronary artery disease (CAD). Antioxidant activity of HDL mainly lies in the activity of paraoxonase (PON). This study aimed to investigate the relationships between PON1 L55M and Q192R polymorphisms, and the risks of CAD in patients with hyperlipidemia. METHODS: From January 2014 to January 2016, 244 patients were divided into hyperlipidemia, hyperlipidemia + CAD, and control groups. The hyperlipidemia and hyperlipidemia + CAD groups were designated as the case group. Serum PON1 concentrations were measured using the enzyme-linked immunosorbent assay. After isolating genomic DNA, the PON1 L55M and Q192R genes were amplified by polymerase chain reaction and sequenced. RESULTS: In the case group, the genotypes LM and LL were detected significantly more often than in the control group, as were the alleles R (33.33%, 42.12%) and L (22.78%, 29.11%). The frequency of QR and RR genotypes was significantly higher in the hyperlipidemia + CAD group than in the hyperlipidemia group; the allele R in the hyperlipidemia + CAD group (42.77%) was more frequent than in the hyperlipidemia group (23.78%). The Q192R polymorphism was associated with low serum PON1 concentrations, and the lowest concentration was observed in the 192QR + 192RR genotype (P = 0.03). Logistic regression analysis showed a significant correlation between the 192R allele and smoking (P = 0.03), body mass index (P = 0.02), systolic blood pressure (P = 0.004), total cholesterol (P = 0.03), triglycerides (P = 0.01), HDL (P = 0.004), and low density lipoprotein (P = 0.02). CONCLUSION: The PON1 alleles 192R and 55L are associated with CAD, and the Q192R polymorphism may be a risk factor for CAD.
OBJECTIVE: A decreased plasma high density lipoprotein (HDL) cholesterol level is a strong risk factor for coronary artery disease (CAD). Antioxidant activity of HDL mainly lies in the activity of paraoxonase (PON). This study aimed to investigate the relationships between PON1L55M and Q192R polymorphisms, and the risks of CAD in patients with hyperlipidemia. METHODS: From January 2014 to January 2016, 244 patients were divided into hyperlipidemia, hyperlipidemia + CAD, and control groups. The hyperlipidemia and hyperlipidemia + CAD groups were designated as the case group. Serum PON1 concentrations were measured using the enzyme-linked immunosorbent assay. After isolating genomic DNA, the PON1L55M and Q192R genes were amplified by polymerase chain reaction and sequenced. RESULTS: In the case group, the genotypes LM and LL were detected significantly more often than in the control group, as were the alleles R (33.33%, 42.12%) and L (22.78%, 29.11%). The frequency of QR and RR genotypes was significantly higher in the hyperlipidemia + CAD group than in the hyperlipidemia group; the allele R in the hyperlipidemia + CAD group (42.77%) was more frequent than in the hyperlipidemia group (23.78%). The Q192R polymorphism was associated with low serum PON1 concentrations, and the lowest concentration was observed in the 192QR + 192RR genotype (P = 0.03). Logistic regression analysis showed a significant correlation between the 192R allele and smoking (P = 0.03), body mass index (P = 0.02), systolic blood pressure (P = 0.004), total cholesterol (P = 0.03), triglycerides (P = 0.01), HDL (P = 0.004), and low density lipoprotein (P = 0.02). CONCLUSION: The PON1 alleles 192R and 55L are associated with CAD, and the Q192R polymorphism may be a risk factor for CAD.
Entities:
Keywords:
Coronary artery disease; Gene polymorphism; High density lipoprotein cholesterol; Lipid metabolism; Paraoxonase-1
Authors: Sudeshna Dasgupta; F Yesim Demirci; Amy S Dressen; Amy H Kao; Elisa Y Rhew; Rosalind Ramsey-Goldman; Susan Manzi; Candace M Kammerer; M Ilyas Kamboh Journal: BMC Med Genet Date: 2011-01-11 Impact factor: 2.103
Authors: Debbie A Lawlor; Ian N M Day; Tom R Gaunt; Lesley J Hinks; Patricia J Briggs; Matthew Kiessling; Nick Timpson; George Davey Smith; Shah Ebrahim Journal: BMC Genet Date: 2004-06-23 Impact factor: 2.797
Authors: Bharti Mackness; Judit Marsillach; Robert S Elkeles; Ian F Godsland; Michael D Feher; Michael B Rubens; Marcus D Flather; Steve E Humphries; Jackie Cooper; Mike Mackness Journal: Dis Markers Date: 2012 Impact factor: 3.434