Toni Dobsak1,2, Patrick Heimel1,2,3, Stefan Tangl1,2, Uwe Y Schwarze2,4, Georg Schett5, Reinhard Gruber2,4,6. 1. Core Facility Hard Tissue and Biomaterial Research, Karl Donath Laboratory, School of Dentistry, Medical University of Vienna, Vienna, Austria. 2. Austrian Cluster for Tissue Regeneration, Vienna, Austria. 3. Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria. 4. Department of Oral Biology, School of Dentistry, Medical University of Vienna, Vienna, Austria. 5. Department of Internal Medicine 3, Friedrich Alexander University of Erlangen- Nuremberg, Erlangen, Germany. 6. Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
Abstract
AIM: Tumour necrosis factor (TNF)-α is a pathological factor causing the characteristic symptoms of periodontal disease and rheumatoid arthritis. In this study, we describe the phenotypes of human TNF-α transgenic mice (hTNFtg) with respect to their periodontium and the temporomandibular joint (TMJ). MATERIAL AND METHODS: Periodontal structures, TMJ and skull shape of hTNFtg mice and wild-type (WT) littermates were assessed by microcomputed tomography, automated segmentation, geometric morphometrics and histologic ground sections. RESULTS: We show that hTNFtg mice have an eroded lamina dura and reduced periodontal ligament space compared to (WT) littermates. Transgenic mice further exhibit severe destruction of the TMJ. Geometric morphometrics revealed that hTNFtg mice have a more laterally positioned TMJ with a concomitantly enlarged zygomatic process. Mandibular and maxillary teeth occluded properly. CONCLUSIONS: Our findings suggest that chronic inflammation in hTNFtg mice causes destructive changes of the periodontium and the TMJ.
AIM: Tumour necrosis factor (TNF)-α is a pathological factor causing the characteristic symptoms of periodontal disease and rheumatoid arthritis. In this study, we describe the phenotypes of human TNF-α transgenic mice (hTNFtg) with respect to their periodontium and the temporomandibular joint (TMJ). MATERIAL AND METHODS: Periodontal structures, TMJ and skull shape of hTNFtg mice and wild-type (WT) littermates were assessed by microcomputed tomography, automated segmentation, geometric morphometrics and histologic ground sections. RESULTS: We show that hTNFtg mice have an eroded lamina dura and reduced periodontal ligament space compared to (WT) littermates. Transgenic mice further exhibit severe destruction of the TMJ. Geometric morphometrics revealed that hTNFtg mice have a more laterally positioned TMJ with a concomitantly enlarged zygomatic process. Mandibular and maxillary teeth occluded properly. CONCLUSIONS: Our findings suggest that chronic inflammation in hTNFtg mice causes destructive changes of the periodontium and the TMJ.
Authors: Karol Alí Apaza Alccayhuaman; Stefan Tangl; Stéphane Blouin; Markus A Hartmann; Patrick Heimel; Ulrike Kuchler; Jung-Seok Lee; Reinhard Gruber Journal: Biomedicines Date: 2021-06-25