AIM: The aim of this study was to evaluate the potential possibility of crude larval and recombinant (rHcp26/23) antigens of Haemonchus contortus for immunization to control sheep hemonchosis. MATERIALS AND METHODS: A total of 21 lambs were divided into five groups. Lambs were immunized with larval and recombinant (rHcp26/23) proteins at day 0 and day 14 and after that challenged with 5000 infective larvae of H. contortus on day 42. An unvaccinated positive control group was challenged with L3 in the meantime. An unvaccinated negative control group was not challenged. RESULTS: Fecal egg count reduction taking after challenge for rHcp26/23 and larval antigens was 92.2% and 38.2%, respectively, compared with the positive control group. Vaccine incited protection in rHcp26/23 and larval immunization was reflected in significant (p<0.05) decreases in worm burden; 59.9% and 40.1%, respectively. CONCLUSION: Recombinant rHcp26/23 vaccine induced a partial immune response and had immune-protective effect against sheep hemonchosis.
AIM: The aim of this study was to evaluate the potential possibility of crude larval and recombinant (rHcp26/23) antigens of Haemonchus contortus for immunization to control sheep hemonchosis. MATERIALS AND METHODS: A total of 21 lambs were divided into five groups. Lambs were immunized with larval and recombinant (rHcp26/23) proteins at day 0 and day 14 and after that challenged with 5000 infective larvae of H. contortus on day 42. An unvaccinated positive control group was challenged with L3 in the meantime. An unvaccinated negative control group was not challenged. RESULTS: Fecal egg count reduction taking after challenge for rHcp26/23 and larval antigens was 92.2% and 38.2%, respectively, compared with the positive control group. Vaccine incited protection in rHcp26/23 and larval immunization was reflected in significant (p<0.05) decreases in worm burden; 59.9% and 40.1%, respectively. CONCLUSION: Recombinant rHcp26/23 vaccine induced a partial immune response and had immune-protective effect against sheep hemonchosis.
Authors: L García-Coiradas; F Angulo-Cubillán; S Méndez; V Larraga; C de la Fuente; M Cuquerella; J M Alunda Journal: Parasitol Res Date: 2008-09-30 Impact factor: 2.289
Authors: Leticia García-Coiradas; Francisco Angulo-Cubillán; Basilio Valladares; Enrique Martínez; Concepción de la Fuente; José María Alunda; Montserrat Cuquerella Journal: Vet Med Int Date: 2010-06-29
Authors: David Piedrafita; Sarah Preston; Joanna Kemp; Michael de Veer; Jayne Sherrard; Troy Kraska; Martin Elhay; Els Meeusen Journal: PLoS One Date: 2013-10-21 Impact factor: 3.240
Authors: Brett Roberts; Aristotelis Antonopoulos; Stuart M Haslam; Alison J Dicker; Tom N McNeilly; Stephanie L Johnston; Anne Dell; David P Knox; Collette Britton Journal: Vet Res Date: 2013-12-01 Impact factor: 3.683