Literature DB >> 28828657

Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC).

Tao Huang1, Jiang He2.   

Abstract

Extracellular vesicles (EVs) have recently attracted substantial attention due to the potential diagnostic and therapeutic relevance. Although a variety of techniques have been used to isolate and analyze EVs, it is still far away from satisfaction. Size-exclusion chromatography (SEC), which separates subjects by size, has been widely applied in protein purification and analysis. The purpose of this chapter is to show the applications of size-exclusion high-performance liquid chromatography (HPLC) as methods for EV characterization of impurities or contaminants of small size, and thus for quality assay for the purity of the samples of EVs.

Entities:  

Keywords:  Exosomes; Extracellular vesicles; High-performance liquid chromatography (HPLC); Size-exclusion chromatography

Mesh：

Substances：

Year:  2017        PMID： 28828657      PMCID： PMC6802587          DOI： 10.1007/978-1-4939-7253-1_15

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  26 in total

Review 1.  Exosomes: composition, biogenesis and function.

Authors:  Clotilde Théry; Laurence Zitvogel; Sebastian Amigorena
Journal:  Nat Rev Immunol       Date:  2002-08       Impact factor: 53.106

2.  Isolation and characterization of exosomes from cell culture supernatants and biological fluids.

Authors:  Clotilde Théry; Sebastian Amigorena; Graça Raposo; Aled Clayton
Journal:  Curr Protoc Cell Biol       Date:  2006-04

3.  Analysis of antigen presenting cell derived exosomes, based on immuno-magnetic isolation and flow cytometry.

Authors:  A Clayton; J Court; H Navabi; M Adams; M D Mason; J A Hobot; G R Newman; B Jasani
Journal:  J Immunol Methods       Date:  2001-01-01       Impact factor: 2.303

Review 4.  A comprehensive overview of exosomes as drug delivery vehicles - endogenous nanocarriers for targeted cancer therapy.

Authors:  Kasper Bendix Johnsen; Johann Mar Gudbergsson; Martin Najbjerg Skov; Linda Pilgaard; Torben Moos; Meg Duroux
Journal:  Biochim Biophys Acta       Date:  2014-04-18

5.  Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma.

Authors:  Jason D Arroyo; John R Chevillet; Evan M Kroh; Ingrid K Ruf; Colin C Pritchard; Donald F Gibson; Patrick S Mitchell; Christopher F Bennett; Era L Pogosova-Agadjanyan; Derek L Stirewalt; Jonathan F Tait; Muneesh Tewari
Journal:  Proc Natl Acad Sci U S A       Date:  2011-03-07       Impact factor: 11.205

Review 6.  Exosome lipidomics unravels lipid sorting at the level of multivesicular bodies.

Authors:  Caroline Subra; Karine Laulagnier; Bertrand Perret; Michel Record
Journal:  Biochimie       Date:  2006-11-16       Impact factor: 4.079

7.  Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes.

Authors:  Javier Conde-Vancells; Eva Rodriguez-Suarez; Nieves Embade; David Gil; Rune Matthiesen; Mikel Valle; Felix Elortza; Shelly C Lu; Jose M Mato; Juan M Falcon-Perez
Journal:  J Proteome Res       Date:  2008-12       Impact factor: 4.466

Review 8.  Exosomes in tumor microenvironment influence cancer progression and metastasis.

Authors:  Christoph Kahlert; Raghu Kalluri
Journal:  J Mol Med (Berl)       Date:  2013-03-22       Impact factor: 4.599

9.  Therapeutic MSC exosomes are derived from lipid raft microdomains in the plasma membrane.

Authors:  Soon Sim Tan; Yijun Yin; Tricia Lee; Ruenn Chai Lai; Ronne Wee Yeh Yeo; Bin Zhang; Andre Choo; Sai Kiang Lim
Journal:  J Extracell Vesicles       Date:  2013-12-23

Review 10.  Exosomal miRNAs as cancer biomarkers and therapeutic targets.

Authors:  Arron Thind; Clive Wilson
Journal:  J Extracell Vesicles       Date:  2016-07-19
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  5 in total

1.  Cartilage Repair by Mesenchymal Stem Cell-Derived Exosomes: Preclinical and Clinical Trial Update and Perspectives.

Authors:  Shahrbano Jahangir; Mojtaba Khozaei Ravari; Leila Taghiyar; Mohammad Amin Shamekhi; Mohamadreza Baghaban Eslaminejad
Journal:  Adv Exp Med Biol       Date:  2021       Impact factor: 2.622

Review 2.  The role of extracellular vesicles from different origin in the microenvironment of head and neck cancers.

Authors:  Changqing Xie; Ning Ji; Zhangui Tang; Jing Li; Qianming Chen
Journal:  Mol Cancer       Date:  2019-04-06       Impact factor: 27.401

Review 3.  Manufacturing Therapeutic Exosomes: from Bench to Industry.

Authors:  So-Hee Ahn; Seung-Wook Ryu; Hojun Choi; Sangmin You; Jun Park; Chulhee Choi
Journal:  Mol Cells       Date:  2022-05-31       Impact factor: 5.034

4.  Eimeria falciformis secretes extracellular vesicles to modulate proinflammatory response during interaction with mouse intestinal epithelial cells.

Authors:  Joshua Seun Olajide; Ling Xiong; Shunli Yang; Zigang Qu; Xiao Xu; Bin Yang; Jing Wang; Baohong Liu; Xueting Ma; Jianping Cai
Journal:  Parasit Vectors       Date:  2022-07-08       Impact factor: 4.047

Review 5.  Multi-Omics Data Integration in Extracellular Vesicle Biology-Utopia or Future Reality?

Authors:  Leona Chitoiu; Alexandra Dobranici; Mihaela Gherghiceanu; Sorina Dinescu; Marieta Costache
Journal:  Int J Mol Sci       Date:  2020-11-13       Impact factor: 5.923
  5 in total

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