Literature DB >> 28828654

Extracellular Vesicle Isolation and Analysis by Western Blotting.

Emma J K Kowal1,2, Dmitry Ter-Ovanesyan3,4,5,6, Aviv Regev7,8, George M Church1,2.   

Abstract

Extracellular vesicles (EVs) are released by mammalian cells and are thought to be important mediators of intercellular communication. There are many methods for isolating EVs from cell culture media, but the most popular methods involve purification based on ultracentrifugation . Here, we provide a detailed protocol for isolating EVs by differential ultracentrifugation and analyzing EV proteins (such as the tetraspanins CD9 , CD63 and CD81 ) by western blotting.

Entities:  

Keywords:  CD63; CD81; CD9; Exosome isolation; Exosomes; Extracellular vesicle isolation; Extracellular vesicles; Immunoblotting; Tetraspanins; Ultracentrifugation; Western blot

Mesh：

Year:  2017        PMID： 28828654     DOI： 10.1007/978-1-4939-7253-1_12

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  27 in total

Review 1.  Extracellular membrane vesicles in the three domains of life and beyond.

Authors:  Sukhvinder Gill; Ryan Catchpole; Patrick Forterre
Journal:  FEMS Microbiol Rev       Date:  2019-05-01       Impact factor: 16.408

Review 2.  Recent advances in nanomaterial-based biosensors for the detection of exosomes.

Authors:  Linan Zhang; Chunchuan Gu; Jiajun Wen; Guangxian Liu; Hongying Liu; Lihua Li
Journal:  Anal Bioanal Chem       Date:  2020-11-08       Impact factor: 4.142

3.  Mesenchymal stem cells and cell-derived extracellular vesicles protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers.

Authors:  Mariana A de Godoy; Leonardo M Saraiva; Luiza R P de Carvalho; Andreia Vasconcelos-Dos-Santos; Hellen J V Beiral; Alane Bernardo Ramos; Livian R de Paula Silva; Renata B Leal; Victor H S Monteiro; Carolina V Braga; Carlla A de Araujo-Silva; Leandro C Sinis; Victor Bodart-Santos; Tais Hanae Kasai-Brunswick; Carolina de Lima Alcantara; Ana Paula C A Lima; Narcisa L da Cunha-E Silva; Antonio Galina; Adalberto Vieyra; Fernanda G De Felice; Rosalia Mendez-Otero; Sergio T Ferreira
Journal:  J Biol Chem       Date:  2017-12-28       Impact factor: 5.157

4.  Mesenchymal stem cell-derived extracellular vesicles and retinal ischemia-reperfusion.

Authors:  Biji Mathew; Sriram Ravindran; Xiaorong Liu; Leianne Torres; Mohansrinivas Chennakesavalu; Chun-Chieh Huang; Liang Feng; Ruth Zelka; Jasmine Lopez; Monica Sharma; Steven Roth
Journal:  Biomaterials       Date:  2019-01-09       Impact factor: 12.479

5.  [Effect of outer membrane vesicles derived from Escherichia coli on proliferation, apoptosis and migration of human neuroblastoma SK-N-SH cells in vitro].

Authors:  Bo Tang; Da-Wei He; Dian Li; Wen-Hao Guo; Dan Zhang; Guang-Hui Wei
Journal:  Nan Fang Yi Ke Da Xue Xue Bao       Date:  2018-03-20

Review 6.  Advances of exosome isolation techniques in lung cancer.

Authors:  Elham O Mahgoub; Ehsan Razmara; Amirreza Bitaraf; Fahimeh-Sadat Norouzi; Maryam Montazeri; Roudabeh Behzadi-Andouhjerdi; Mojtaba Falahati; Ke Cheng; Yousif Haik; Anwarul Hasan; Sadegh Babashah
Journal:  Mol Biol Rep       Date:  2020-08-12       Impact factor: 2.316

7.  uPAR+ extracellular vesicles: a robust biomarker of resistance to checkpoint inhibitor immunotherapy in metastatic melanoma patients.

Authors:  Letizia Porcelli; Michele Guida; Simona Serratì; Amalia Azzariti; Simona De Summa; Roberta Di Fonte; Ivana De Risi; Marianna Garofoli; Mariapia Caputo; Antonio Negri; Sabino Strippoli
Journal:  J Immunother Cancer       Date:  2021-05       Impact factor: 13.751

8.  Exosomes are secreted at similar densities by M21 and PC3 human cancer cells and show paclitaxel solubility.

Authors:  William S Fisher; Christine Tchounwou; Sophia Wei; Logan Roberts; Kai K Ewert; Cyrus R Safinya
Journal:  Biochim Biophys Acta Biomembr       Date:  2021-12-22       Impact factor: 3.747

9.  The Interaction between Reactive Peritoneal Mesothelial Cells and Tumor Cells via Extracellular Vesicles Facilitates Colorectal Cancer Dissemination.

Authors:  Simona Serratì; Letizia Porcelli; Francesco Fragassi; Marianna Garofoli; Roberta Di Fonte; Livia Fucci; Rosa Maria Iacobazzi; Antonio Palazzo; Francesca Margheri; Grazia Cristiani; Anna Albano; Raffaele De Luca; Donato Francesco Altomare; Michele Simone; Amalia Azzariti
Journal:  Cancers (Basel)       Date:  2021-05-20       Impact factor: 6.639

10.  Hypoxic ADSCs-derived EVs promote the proliferation and chondrogenic differentiation of cartilage stem/progenitor cells.

Authors:  Ke Xue; Yongkang Jiang; Xiaodie Zhang; Jun Wu; Lin Qi; Kai Liu
Journal:  Adipocyte       Date:  2021-12       Impact factor: 4.534
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