Literature DB >> 28828651

Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification.

Richard Lobb1, Andreas Möller2,3.   

Abstract

Exosomes were originally described 29 years ago as a mechanism for the removal of redundant molecules from reticulocytes, nothing but a process of removing cellular trash. It is now however, abundantly clear that exosomes have a more significant biological role. However, there is currently limited information pertaining to efficient isolation procedures that can be used to isolate exosomes from both cell culture media, and clinical samples such as plasma. Here, we present a reliable and efficient procedure that can be utilized for the isolation of exosomes from various starting materials.

Entities:  

Keywords:  Cell culture supernatant; Exosomes; Extracellular vesicles; Human plasma; Isolation

Mesh:

Substances:

Year:  2017        PMID: 28828651     DOI: 10.1007/978-1-4939-7253-1_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  14 in total

1.  Construction of Yeast Display Libraries for Selection of Antigen-Binding Variants of Large Extracellular Loop of CD81, a Major Surface Marker Protein of Extracellular Vesicles.

Authors:  Stefan Vogt; Gerhard Stadlmayr; Katharina Stadlbauer; Florian Stracke; Madhusudhan Reddy Bobbili; Johannes Grillari; Florian Rüker; Gordana Wozniak-Knopp
Journal:  Methods Mol Biol       Date:  2022

Review 2.  Potential theranostics of circulating tumor cells and tumor-derived exosomes application in colorectal cancer.

Authors:  Somayeh Vafaei; Raheleh Roudi; Zahra Madjd; Amir Reza Aref; Marzieh Ebrahimi
Journal:  Cancer Cell Int       Date:  2020-07-06       Impact factor: 5.722

Review 3.  Exosomal biomarkers for cancer diagnosis and patient monitoring.

Authors:  Amy Makler; Waseem Asghar
Journal:  Expert Rev Mol Diagn       Date:  2020-02-20       Impact factor: 5.225

Review 4.  Unraveling the mechanisms that specify molecules for secretion in extracellular vesicles.

Authors:  Andrew M Leidal; Jayanta Debnath
Journal:  Methods       Date:  2020-01-21       Impact factor: 3.608

5.  Exploring the molecular content of CHO exosomes during bioprocessing.

Authors:  Christoph Keysberg; Oliver Hertel; Louise Schelletter; Tobias Busche; Chiara Sochart; Jörn Kalinowski; Raimund Hoffrogge; Kerstin Otte; Thomas Noll
Journal:  Appl Microbiol Biotechnol       Date:  2021-05-03       Impact factor: 4.813

Review 6.  Extracellular Vesicles in Musculoskeletal Pathologies and Regeneration.

Authors:  Marietta Herrmann; Solvig Diederichs; Svitlana Melnik; Jana Riegger; Drenka Trivanović; Shushan Li; Zsuzsa Jenei-Lanzl; Rolf E Brenner; Markus Huber-Lang; Frank Zaucke; Frank A Schildberg; Susanne Grässel
Journal:  Front Bioeng Biotechnol       Date:  2021-01-20

Review 7.  Roles of Exosomes in Ocular Diseases.

Authors:  Jia Liu; Feng Jiang; Yu Jiang; Yicheng Wang; Zelin Li; Xuefeng Shi; Yanping Zhu; Hongbo Wang; Zhuhong Zhang
Journal:  Int J Nanomedicine       Date:  2020-12-29

Review 8.  Message in a Bottle: Upgrading Cardiac Repair into Rejuvenation.

Authors:  Carolina Balbi; Ambra Costa; Lucio Barile; Sveva Bollini
Journal:  Cells       Date:  2020-03-15       Impact factor: 6.600

9.  Highly purified extracellular vesicles from human cardiomyocytes demonstrate preferential uptake by human endothelial cells.

Authors:  Limor Zwi-Dantsis; Charles W Winter; Ulrike Kauscher; Arianna Ferrini; Brian Wang; Thomas E Whittaker; Steve R Hood; Cesare M Terracciano; Molly M Stevens
Journal:  Nanoscale       Date:  2020-09-24       Impact factor: 7.790

Review 10.  A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?

Authors:  Karim Sidhom; Patience O Obi; Ayesha Saleem
Journal:  Int J Mol Sci       Date:  2020-09-04       Impact factor: 5.923

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