Matthias Aurich1,2,3, Gunther Olaf Hofmann4, Bernd Rolauffs5,6. 1. Center of Orthopaedic and Trauma Surgery, Klinikum Ingolstadt, Krumenauerstr. 25, 85049, Ingolstadt, Germany. kontakt@matthias-aurich.de. 2. Department of Trauma, Hand and Reconstructive Surgery, University Hospital Jena, Erlanger Allee 101, 07747, Jena, Germany. kontakt@matthias-aurich.de. 3. Department of Biochemistry, Rush Medical College, 1735 W. Harrison St., Chicago, IL, 60612, USA. kontakt@matthias-aurich.de. 4. Department of Trauma, Hand and Reconstructive Surgery, University Hospital Jena, Erlanger Allee 101, 07747, Jena, Germany. 5. G.E.R.N. Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Medical Center - Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106, Freiburg, Germany. 6. Massachusetts Institute of Technology, Center for Biomedical Engineering, 500 Technology Sq, Cambridge, MA, 02139, USA.
Abstract
PURPOSE: The aim was to analyse the biological characteristics of chondrocytes from the two biopsy sites notch vs. trochlea of human knee joints. The question was whether tissue engineering-relevant characteristics such as viability and mRNA expression profile would be comparable ex vivo and after monolayer expansion, as these are parts of routine autologous chondrocyte implantation (ACI). METHODS: Biopsies from the intercondylar notch and the lateral aspect of the trochlea from 20 patients with ICRS grades 3 and 4 cartilage defects were harvested during arthroscopy. Collagen types 1, 2, and 10 mRNA were quantified by polymerase chain reaction. RESULTS: Compared with notch chondrocytes, ex vivo trochlea chondrocytes had comparable cell numbers, vitality and aggrecan, collagen types 1, -2 and -10 mRNA expression. After monolayer expansion both notch and trochlea chondrocyte characteristics were comparably altered, regardless of their biopsy origin, and no significant differences in viability and mRNA expression were noted. CONCLUSIONS: Collectively, these findings suggest that tissue engineering-relevant characteristics of notch and trochlea chondrocytes are comparable ex vivo and after monolayer expansion. Thus, trochlea chondrocytes promise clinical potential and chondrocytes for ACI could potentially be generated from both notch and trochlea biopsy sites.
PURPOSE: The aim was to analyse the biological characteristics of chondrocytes from the two biopsy sites notch vs. trochlea of human knee joints. The question was whether tissue engineering-relevant characteristics such as viability and mRNA expression profile would be comparable ex vivo and after monolayer expansion, as these are parts of routine autologous chondrocyte implantation (ACI). METHODS: Biopsies from the intercondylar notch and the lateral aspect of the trochlea from 20 patients with ICRS grades 3 and 4 cartilage defects were harvested during arthroscopy. Collagen types 1, 2, and 10 mRNA were quantified by polymerase chain reaction. RESULTS: Compared with notch chondrocytes, ex vivo trochlea chondrocytes had comparable cell numbers, vitality and aggrecan, collagen types 1, -2 and -10 mRNA expression. After monolayer expansion both notch and trochlea chondrocyte characteristics were comparably altered, regardless of their biopsy origin, and no significant differences in viability and mRNA expression were noted. CONCLUSIONS: Collectively, these findings suggest that tissue engineering-relevant characteristics of notch and trochlea chondrocytes are comparable ex vivo and after monolayer expansion. Thus, trochlea chondrocytes promise clinical potential and chondrocytes for ACI could potentially be generated from both notch and trochlea biopsy sites.
Authors: Wolfgang Eger; Matthias Aurich; Barbara L Schumacher; Jürgen Mollenhauer; Klaus E Kuettner; Ada A Cole Journal: Z Orthop Ihre Grenzgeb Date: 2003 Jan-Feb
Authors: Stefan Marlovits; Philip Zeller; Philipp Singer; Christoph Resinger; Vilmos Vécsei Journal: Eur J Radiol Date: 2005-09-26 Impact factor: 3.528
Authors: Matthias Aurich; Harvinder S Bedi; Peter J Smith; Bernd Rolauffs; Thomas Mückley; James Clayton; Mark Blackney Journal: Am J Sports Med Date: 2010-11-10 Impact factor: 6.202
Authors: P Niemeyer; S Andereya; P Angele; A Ateschrang; M Aurich; M Baumann; P Behrens; U Bosch; C Erggelet; S Fickert; J Fritz; H Gebhard; K Gelse; D Günther; A Hoburg; P Kasten; T Kolombe; H Madry; S Marlovits; N M Meenen; P E Müller; U Nöth; J P Petersen; M Pietschmann; W Richter; B Rolauffs; K Rhunau; B Schewe; A Steinert; M R Steinwachs; G H Welsch; W Zinser; D Albrecht Journal: Z Orthop Unfall Date: 2013-02-19 Impact factor: 0.923
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