OBJECTIVE: The prevalence of celiac disease (CD) has increased significantly in recent years, and risk prediction and early diagnosis have become imperative especially in at-risk families. In a previous study, we identified individuals with CD based on the expression profile of a set of candidate genes in peripheral blood monocytes. Here we evaluated the expression of a panel of CD candidate genes in peripheral blood mononuclear cells from at-risk infants long time before any symptom or production of antibodies. METHODS: We analyzed the gene expression of a set of 9 candidate genes, associated with CD, in 22 human leukocyte antigen predisposed children from at-risk families for CD, studied from birth to 6 years of age. Nine of them developed CD (patients) and 13 did not (controls). We analyzed gene expression at 3 different time points (age matched in the 2 groups): 4-19 months before diagnosis, at the time of CD diagnosis, and after at least 1 year of a gluten-free diet. At similar age points, controls were also evaluated. RESULTS: Three genes (KIAA, TAGAP [T-cell Activation GTPase Activating Protein], and SH2B3 [SH2B Adaptor Protein 3]) were overexpressed in patients, compared with controls, at least 9 months before CD diagnosis. At a stepwise discriminant analysis, 4 genes (RGS1 [Regulator of G-protein signaling 1], TAGAP, TNFSF14 [Tumor Necrosis Factor (Ligand) Superfamily member 14], and SH2B3) differentiate patients from controls before serum antibodies production and clinical symptoms. Multivariate equation correctly classified CD from non-CD children in 95.5% of patients. CONCLUSIONS: The expression of a small set of candidate genes in peripheral blood mononuclear cells can predict CD at least 9 months before the appearance of any clinical and serological signs of the disease.
OBJECTIVE: The prevalence of celiac disease (CD) has increased significantly in recent years, and risk prediction and early diagnosis have become imperative especially in at-risk families. In a previous study, we identified individuals with CD based on the expression profile of a set of candidate genes in peripheral blood monocytes. Here we evaluated the expression of a panel of CD candidate genes in peripheral blood mononuclear cells from at-risk infants long time before any symptom or production of antibodies. METHODS: We analyzed the gene expression of a set of 9 candidate genes, associated with CD, in 22 human leukocyte antigen predisposed children from at-risk families for CD, studied from birth to 6 years of age. Nine of them developed CD (patients) and 13 did not (controls). We analyzed gene expression at 3 different time points (age matched in the 2 groups): 4-19 months before diagnosis, at the time of CD diagnosis, and after at least 1 year of a gluten-free diet. At similar age points, controls were also evaluated. RESULTS: Three genes (KIAA, TAGAP [T-cell Activation GTPase Activating Protein], and SH2B3 [SH2B Adaptor Protein 3]) were overexpressed in patients, compared with controls, at least 9 months before CD diagnosis. At a stepwise discriminant analysis, 4 genes (RGS1 [Regulator of G-protein signaling 1], TAGAP, TNFSF14 [Tumor Necrosis Factor (Ligand) Superfamily member 14], and SH2B3) differentiate patients from controls before serum antibodies production and clinical symptoms. Multivariate equation correctly classified CD from non-CD children in 95.5% of patients. CONCLUSIONS: The expression of a small set of candidate genes in peripheral blood mononuclear cells can predict CD at least 9 months before the appearance of any clinical and serological signs of the disease.
Authors: Allie B Cichewicz; Elizabeth S Mearns; Aliki Taylor; Talia Boulanger; Michele Gerber; Daniel A Leffler; Jennifer Drahos; David S Sanders; Kelly J Thomas Craig; Benjamin Lebwohl Journal: Dig Dis Sci Date: 2019-03-01 Impact factor: 3.199
Authors: D Cielo; M Galatola; N Fernandez-Jimenez; L De Leo; K Garcia-Etxebarria; C Loganes; A Tommasini; T Not; R Auricchio; L Greco; J R Bilbao Journal: Sci Rep Date: 2019-07-10 Impact factor: 4.379
Authors: R Auricchio; M Galatola; D Cielo; A Amoresano; M Caterino; E De Vita; A Illiano; R Troncone; L Greco; M Ruoppolo Journal: Sci Rep Date: 2019-10-04 Impact factor: 4.379
Authors: Aarón D Ramírez-Sánchez; Ineke L Tan; B C Gonera-de Jong; Marijn C Visschedijk; Iris Jonkers; Sebo Withoff Journal: Int J Mol Sci Date: 2020-11-12 Impact factor: 5.923
Authors: Ineke L Tan; Rodrigo Coutinho de Almeida; Rutger Modderman; Anna Stachurska; Jackie Dekens; Donatella Barisani; Caroline R Meijer; María Roca; Eva Martinez-Ojinaga; Raanan Shamir; Renata Auricchio; Ilma R Korponay-Szabó; Gemma Castillejo; Hania Szajewska; Sibylle Koletzko; Alexandra Zhernakova; Vinod Kumar; Yang Li; Marijn C Visschedijk; Rinse K Weersma; Riccardo Troncone; M Luisa Mearin; Cisca Wijmenga; Iris Jonkers; Sebo Withoff Journal: Front Immunol Date: 2021-12-07 Impact factor: 7.561