Fábio P Sellera1, Miriam R Fernandes2, Quézia Moura3, Tiago A Souza4, Louise Cerdeira2, Nilton Lincopan5. 1. Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil. 2. Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil. 3. Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. 4. Genome Investigation and Analysis Laboratory (GENIAL), Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. 5. Department of Clinical Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil. Electronic address: lincopan@usp.br.
Abstract
OBJECTIVES: Aquatic environments have contributed to the dissemination of multidrug-resistant (MDR) bacteria, representing a risk for humans and animals. The aim of this study was to report the first draft genome sequence of a MDR Enterobacter cloacae strain recovered from seawater in a public beach in Brazil. METHODS: The genome was sequenced on an Illumina MiSeq platform. De novo genome assembly was performed using SPAdes 3.10.1 and the whole genome sequence was analysed using bioinformatics tools from the Center of Genomic Epidemiology. RESULTS: This draft genome resulted in 5 228 857bp with 5331 protein-coding sequences, revealing the presence of blaKPC-2, blaCTX-M-15 and blaOXA-17 genes, responsible for resistance to all β-lactam antibiotics. In addition, the strain was assigned to sequenced type 520 (ST520). CONCLUSION: These data provide useful information for comparative genomic analysis regarding the dissemination of antibiotic resistance genes.
OBJECTIVES: Aquatic environments have contributed to the dissemination of multidrug-resistant (MDR) bacteria, representing a risk for humans and animals. The aim of this study was to report the first draft genome sequence of a MDR Enterobacter cloacae strain recovered from seawater in a public beach in Brazil. METHODS: The genome was sequenced on an Illumina MiSeq platform. De novo genome assembly was performed using SPAdes 3.10.1 and the whole genome sequence was analysed using bioinformatics tools from the Center of Genomic Epidemiology. RESULTS: This draft genome resulted in 5 228 857bp with 5331 protein-coding sequences, revealing the presence of blaKPC-2, blaCTX-M-15 and blaOXA-17 genes, responsible for resistance to all β-lactam antibiotics. In addition, the strain was assigned to sequenced type 520 (ST520). CONCLUSION: These data provide useful information for comparative genomic analysis regarding the dissemination of antibiotic resistance genes.
Authors: Fábio P Sellera; Brenda Cardoso; Danny Fuentes-Castillo; Fernanda Esposito; Elder Sano; Herrison Fontana; Bruna Fuga; Daphne W Goldberg; Lourdes A V Seabra; Marzia Antonelli; Sandro Sandri; Cristiane K M Kolesnikovas; Nilton Lincopan Journal: Front Microbiol Date: 2022-06-10 Impact factor: 6.064
Authors: Vanessa Bueris; Fábio P Sellera; Bruna Fuga; Elder Sano; Marcelo P N Carvalho; Samuel C F Couto; Quézia Moura; Nilton Lincopan Journal: Sci Rep Date: 2022-04-05 Impact factor: 4.379
Authors: Yasir M Bashawri; Peter Robins; David M Cooper; James E McDonald; Davey L Jones; A Prysor Williams Journal: Int J Environ Res Public Health Date: 2020-10-19 Impact factor: 3.390