New insights into the impacts of elevated CO2, nitrogen, and temperature levels on the regulation of C and N metabolism in durum wheat using network analysis.

The use of correlation networks and hierarchical cluster analysis provides a framework to organize and study the coordination of parameters such as genes, metabolites, proteins and physiological parameters. We have analyzed 142 traits from primary C and N metabolism, including biochemical and gene expression analyses, in a range of 32 different growth conditions (various [CO2] levels, temperatures, N supplies, growth stages and experimental methods). To test the integration of primary metabolism, particularly under climate change, we investigated which C and N metabolic traits and transcript levels are correlated in durum wheat flag leaves using a correlation network and a hierarchical cluster analysis. There was a high amount of positive correlation between traits involved in a wide range of biological processes, suggesting a close and intricate coordination between C-N metabolisms at the biochemical and transcriptional levels. Transcript levels for genes related to N uptake and assimilation were especially coexpressed with genes belonging to the respiratory pathway, highlighting the coordination between the synthesis of organic N compounds and provision of energy and C skeletons. Also involved in this coordination were Rubisco and nitrate reductase activities, which play a key role in the regulation of plant metabolism. Carbohydrate accumulation was linked with a down-regulation of photosynthetic and N metabolism genes and nitrate reductase activity. Based on the degree of connectivity between nodes, network exploration facilitated the identification of some traits that may be biologically relevant during plant abiotic stress tolerance, as most of them are involved in limiting steps of plant metabolism.