Literature DB >> 28826394

Erratum to: Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phage.

Martin Ploss1, Andreas Kuhn2.   

Abstract

Entities:  

Year:  2017        PMID: 28826394      PMCID: PMC5567570          DOI: 10.1186/s12866-017-1072-9

Source DB:  PubMed          Journal:  BMC Microbiol        ISSN: 1471-2180            Impact factor:   3.605


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Erratum

After publication of our article [1], we became aware that during the assembly of panel A in Fig. 6 a wrong control lane (α T7) had been included. The results and conclusions are not affected by this error.
Fig. 6

Presentation of the antigenic tags on gp9 of phage particles. (a) M13 phage (panel a) was applied onto nitrocellulose membrane and incubated with antibody to gp8, T7tag and HA tag, respectively, at the indicated concentrations. To visualize the phage a peroxidase-linked secondary antibody was applied and analysed by chemoluminescence. b M13 am9 phage grown in E. coli K38 cells bearing a plasmid encoding gp9-T7 or gp9DT7, respectively, were incubated with antibody to T7 and treated as described above. c M13 am9 phage propagated in E. coli K38 cells bearing a plasmid encoding gp9-HA or gp9DHA, respectively, were incubated with antibody to HA and treated as described above. For controls (Ctr), uninfected cultures were tested under identical conditions.

The corrected Fig. 6 is shown as follows: Presentation of the antigenic tags on gp9 of phage particles. (a) M13 phage (panel a) was applied onto nitrocellulose membrane and incubated with antibody to gp8, T7tag and HA tag, respectively, at the indicated concentrations. To visualize the phage a peroxidase-linked secondary antibody was applied and analysed by chemoluminescence. b M13 am9 phage grown in E. coli K38 cells bearing a plasmid encoding gp9-T7 or gp9DT7, respectively, were incubated with antibody to T7 and treated as described above. c M13 am9 phage propagated in E. coli K38 cells bearing a plasmid encoding gp9-HA or gp9DHA, respectively, were incubated with antibody to HA and treated as described above. For controls (Ctr), uninfected cultures were tested under identical conditions.
  1 in total

1.  Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phage.

Authors:  Martin Ploss; Andreas Kuhn
Journal:  BMC Microbiol       Date:  2011-09-26       Impact factor: 3.605

  1 in total

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