Xinying Wang1, Jianbo Yang2, Feng Tian3, Li Zhang4, Qiucheng Lei5, Tingting Jiang6, Jihong Zhou7, Siming Yuan8, Jun Wang9, Zhijian Feng10, Jieshou Li11. 1. Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. Electronic address: wangxyjlh@126.com. 2. Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. Electronic address: yang_jianbo@126.com. 3. Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. Electronic address: tianfeng_nju@163.com. 4. Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. Electronic address: zlshe1107@163.com. 5. Department of General Surgery, The First People's Hospital of Foshan, Sun Yat-Sen University, Foshan 528000, China. Electronic address: lqiuchenggd@163.com. 6. Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China. Electronic address: jiangtingting08med@163.com. 7. Department of Burn, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. Electronic address: zhoujhjlh@126.com. 8. Department of Burn, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. Electronic address: yuansmjlh@126.com. 9. Department of Burn, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. Electronic address: wangj_jlh@126.com. 10. Department of Burn, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. Electronic address: fengzj_jlh@126.com. 11. Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. Electronic address: lijsnjujlh@126.com.
Abstract
PURPOSE: This time series experiments aimed to investigate the dynamic change of gut microbiomes after severe burn and its association with enteral nutrition (EN). MATERIALS AND METHODS: Seven severely burned patients who suffered from a severe metal dust explosion injury were recruited in this study. The dynamic changes of gut microbiome of fecal samples at six time points (1-3days, 2, 3, 4, 5 and 6weeks after severe burn) were detected using 16S ribosomal RNA pyrosequencing technology. RESULTS: Following the post-burn temporal order, gut microbiota dysbiosis was detected in the gut microbiome after severe burn, then it was gradually resolved. The bio-diversity of gut bacteria was initially decreased, and then returned to normal level. In addition, at the early stage (from 2 to 4weeks), the majority of those patients' gut microbiome were opportunistic pathogen genus, Enterococcus and Escherichia; while at the end of this study, the majority was a beneficial genus, Bacteroides. EN can promote the recovery of gut microbiota, especially in EN well-tolerated patients. CONCLUSIONS: Severe burn injury can cause a dramatic dysbiosis of gut microbiota. A trend of enriched beneficial bacteria and diminished opportunistic pathogen bacteria may serve as prognosis microbiome biomarkers of severe burn patients.
PURPOSE: This time series experiments aimed to investigate the dynamic change of gut microbiomes after severe burn and its association with enteral nutrition (EN). MATERIALS AND METHODS: Seven severely burned patients who suffered from a severe metal dust explosion injury were recruited in this study. The dynamic changes of gut microbiome of fecal samples at six time points (1-3days, 2, 3, 4, 5 and 6weeks after severe burn) were detected using 16S ribosomal RNA pyrosequencing technology. RESULTS: Following the post-burn temporal order, gut microbiota dysbiosis was detected in the gut microbiome after severe burn, then it was gradually resolved. The bio-diversity of gut bacteria was initially decreased, and then returned to normal level. In addition, at the early stage (from 2 to 4weeks), the majority of those patients' gut microbiome were opportunistic pathogen genus, Enterococcus and Escherichia; while at the end of this study, the majority was a beneficial genus, Bacteroides. EN can promote the recovery of gut microbiota, especially in EN well-tolerated patients. CONCLUSIONS: Severe burn injury can cause a dramatic dysbiosis of gut microbiota. A trend of enriched beneficial bacteria and diminished opportunistic pathogen bacteria may serve as prognosis microbiome biomarkers of severe burn patients.
Authors: Kiran Dyamenahalli; Kevin Choy; Daniel N Frank; Kevin Najarro; Devin Boe; Kathryn L Colborn; Juan-Pablo Idrovo; Anne L Wagner; Arek J Wiktor; Majid Afshar; Ellen L Burnham; Rachel H McMahan; Elizabeth J Kovacs Journal: J Burn Care Res Date: 2022-09-01 Impact factor: 1.819
Authors: Elizabeth G Wheatley; Brenda J Curtis; Holly J Hulsebus; Devin M Boe; Kevin Najarro; Diana Ir; Charles E Robertson; Mashkoor A Choudhry; Daniel N Frank; Elizabeth J Kovacs Journal: Shock Date: 2020-01 Impact factor: 3.533
Authors: Gloria M Agudelo-Ochoa; Beatriz E Valdés-Duque; Nubia A Giraldo-Giraldo; Ana M Jaillier-Ramírez; Adriana Giraldo-Villa; Irene Acevedo-Castaño; Mónica A Yepes-Molina; Janeth Barbosa-Barbosa; Alfonso Benítez-Paéz Journal: Gut Microbes Date: 2020-01-10