| Literature DB >> 28822138 |
Roxana Khoshravesh1, Vanessa Lundsgaard-Nielsen1, Stefanie Sultmanis1, Tammy L Sage2.
Abstract
High-resolution images obtained from plant tissues processed for light microscopy, transmission electron microscopy, and immunohistochemistry have provided crucial links between plant subcellular structure and physiology during photorespiration as well as the impact of photorespiration on plant evolution and development. This chapter presents established protocols to guide researchers in the preparation of plant tissues for high-resolution imaging with a light and transmission electron microscope and detection of proteins using immunohistochemistry. Discussion of concepts and theory behind each step in the process from tissue preservation to staining of resin-embedded tissues is included to enhance the understanding of all steps in the procedure. We also include a brief protocol for quantification of cellular parameters from high-resolution images to help researchers rigorously test hypotheses.Keywords: Chloroplasts; High-resolution microscopy; Immunohistochemistry; Light microscopy; Mitochondria; Peroxisomes; Photorespiration; Transmission electron microscopy
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Year: 2017 PMID： 28822138 DOI： 10.1007/978-1-4939-7225-8_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745