| Literature DB >> 28822124 |
Abstract
The determination of enzyme activities in organ or organellar extracts is an important means of investigating metabolic networks and allows testing the success of enzyme-targeted genetic engineering. It also delivers information on intrinsic enzyme parameters such as kinetic properties or impact of effector molecules. This chapter provides protocols on how to assess activities of the enzymes of the core photorespiratory pathway, from 2-phosphoglycolate phosphatase to glycerate 3-kinase.Keywords: 2-Phosphoglycolate phosphatase; Enzyme assays; Glycerate 3-kinase; Glycine decarboxylase; Glycolate oxidase; Glyoxylate aminotransferase; Photorespiration; Serine hydroxymethyltransferase
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Year: 2017 PMID： 28822124 DOI： 10.1007/978-1-4939-7225-8_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745