Michinobu Ohno1, Yasushi Fuchimoto2, Huai-Che Hsu3, Masataka Higuchi4, Makoto Komura5, Tetsuji Yamaoka6, Akihiro Umezawa7, Shin Enosawa3, Tatsuo Kuroda8. 1. Division of Surgery, Department of Surgical Specialties, National Center for Child Health and Development, Tokyo, Japan. 2. Department of Pediatric Surgery, Graduate School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan. yfuchimoto@keio.jp. 3. Division for Advanced Medical Sciences, National Center for Child Health and Development, Tokyo, Japan. 4. Division of Pulmonology, Department of Medical Specialties, National Center for Child Health and Development, Tokyo, Japan. 5. Department of Pediatric Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan. 6. Department of Biomedical Engineering, National Cerebral and Cardiovascular Center Research Institute, Suita, Japan. 7. Department of Reproductive Biology, National Center for Child Health and Development, Tokyo, Japan. 8. Department of Pediatric Surgery, Graduate School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan.
Abstract
PURPOSE: Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. METHODS: Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 × 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies. RESULTS: No pigs displayed airway symptoms during the observation period. Tracheal lumen restored by fresh graft patches showed more advanced narrowing than that treated with decellularized grafts by bronchoscopy. Histologically, fresh grafts induced typical cellular rejection; this was decreased with decellularized grafts. In addition, immunohistochemistry demonstrated regenerating foci of recipient cartilage along the adjacent surface of decellularized tracheal grafts. CONCLUSION: Decellularized allogeneic tracheal scaffolds could be effective materials for restoring impaired trachea.
PURPOSE: Tracheal cartilage reconstruction is an essential approach for the treatment of tracheal congenital abnormalities or injury. Here, we evaluated the use of allogeneic decellularized tracheas as novel support scaffolds. METHODS: Six weaned pigs (4-week-old domestic males) were transplanted with allogeneic tracheal graft patches (three decellularized and three fresh tracheal scaffolds) onto artificial defects (approximately 15 × 15 mm). After 11 weeks, the tracheas were evaluated by bronchoscopy and histological studies. RESULTS: No pigs displayed airway symptoms during the observation period. Tracheal lumen restored by fresh graft patches showed more advanced narrowing than that treated with decellularized grafts by bronchoscopy. Histologically, fresh grafts induced typical cellular rejection; this was decreased with decellularized grafts. In addition, immunohistochemistry demonstrated regenerating foci of recipient cartilage along the adjacent surface of decellularized tracheal grafts. CONCLUSION: Decellularized allogeneic tracheal scaffolds could be effective materials for restoring impaired trachea.
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