Green approach for one-pot synthesis of silver nanorod using cellulose nanocrystal and their cytotoxicity and antibacterial assessment.

Herein, this research addresses an innovative approach for one-pot synthesis of highly stabilized silver nanorods in powder form at concentration as high as feasible to be proposed in large-scale production via cellulose nanocrystals (CNC). For the first time, CNC without any surface modification in the presence of alkali is acting as both reducing and stabilizing agent for assembling of Ag nanorods. Extraction of CNC from cotton is carried out as per to acid hydrolysis technique. Thorough assessments of Ag nanorods formation, structural and morphological characteristics of Ag nanorods were investigated by making use of UV-vis spectroscopy, TEM, DLS, AFM and X-ray diffraction (XRD) analysis. Also, the antibacterial activity and cytotoxicity of Ag nanorod were investigated. Research outputs signify that, Ag nanorods has been successfully prepared through an effectively approach by virtue of the textural feature of CNC as a mediator. Results revealed the great tendency of CNC toward reducing and stabilizing the as formed Ag nanorods even at high concentration. Results also demonstrated that Ag nanorods have not merely remarkably antibacterial activity towards Gram-positive and Gram-negative bacteria, but safe for using in human life, which exhibited no effect on eukaryotic cells.