Mariane Maffei Azuma1, João Eduardo Gomes-Filho2, Annelise Katrine Carrara Prieto2, Renata Oliveira Samuel3, Valéria Marçal Felix de Lima4, Dóris Hissako Sumida5, Edilson Ervolino5, Luciano Tavares Angelo Cintra6. 1. Department of Endodontics, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Rua José Bonifácio, 1193, Araçatuba, 16015-050, São Paulo, Brazil; Department of Endodontics, Ingá University Center, UNINGÁ, Rod. PR 317, 6114- Parque Industrial 200, Maringá, Paraná, Brazil. 2. Department of Endodontics, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Rua José Bonifácio, 1193, Araçatuba, 16015-050, São Paulo, Brazil. 3. Department of Endodontics, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Rua José Bonifácio, 1193, Araçatuba, 16015-050, São Paulo, Brazil; Department of Clinical Dentistry, Dental School, UNIUBE, University of Uberaba, Av. Nenê Sabino, 1801, Uberaba, 38055-500, Minas Gerais, Brazil. 4. Department of Clinic and Surgery and Animal Reproduction, São Paulo State University (Unesp), Araçatuba Veterinary Medicine, Rua Clóvis Pestana, 793, Araçatuba, 16050-680, São Paulo, Brazil. 5. Department of Basic Science, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil. 6. Department of Endodontics, São Paulo State University (Unesp), School of Dentistry, Araçatuba, Rua José Bonifácio, 1193, Araçatuba, 16015-050, São Paulo, Brazil. Electronic address: lucianocintra@foa.unesp.br.
Abstract
OBJECTIVES: This study aimed to evaluate the association between endodontic infection and diabetes on interleukin-17 levels in periapical, hepatic, and renal tissues of rats. DESIGN: Forty male rats were divided into groups: normoglycemic rats (N), normoglycemic rats with apical periodontitis (N-AP), rats with experimental diabetes (ED), and rats with experimental diabetes and apical periodontitis (ED-AP). Diabetes was induced by intravenous streptozotocin injection, and blood sugar levels were monitored to confirm disease development. Apical periodontitis (AP) was induced by pulp exposure to the oral environment during 30days. After 30days, hepatic and renal tissues were obtained, and IL-17 levels were quantified by ELISA. The right hemi-jaw was used to quantify IL-17 levels by immunohistochemistry. The values obtained in parametric tests were tabulated and analyzed statistically by analysis of variance (ANOVA) and Tukey tests, and the values obtained for scores were statistically analyzed by using the Kruskal-Wallis and Dun tests. The level of significance was set at 5%. RESULTS: ED and ED-AP groups expressed significantly higher IL-17 levels in both hepatic and renal tissues (p<0.05), compared to N and N-AP groups. Apical periodontitis (AP) in ED-AP group was significantly more severe than that in N-AP group (p<0.05). Furthermore, there was a significantly larger increase in the IL-17 levels in ED-AP group compared to N group (p<0.05). CONCLUSION: Our results indicate that diabetes increases IL-17 levels in hepatic and renal tissues and also enhances IL-17 production in apical periodontitis area of rats.
OBJECTIVES: This study aimed to evaluate the association between endodontic infection and diabetes on interleukin-17 levels in periapical, hepatic, and renal tissues of rats. DESIGN: Forty male rats were divided into groups: normoglycemic rats (N), normoglycemic rats with apical periodontitis (N-AP), rats with experimental diabetes (ED), and rats with experimental diabetes and apical periodontitis (ED-AP). Diabetes was induced by intravenous streptozotocin injection, and blood sugar levels were monitored to confirm disease development. Apical periodontitis (AP) was induced by pulp exposure to the oral environment during 30days. After 30days, hepatic and renal tissues were obtained, and IL-17 levels were quantified by ELISA. The right hemi-jaw was used to quantify IL-17 levels by immunohistochemistry. The values obtained in parametric tests were tabulated and analyzed statistically by analysis of variance (ANOVA) and Tukey tests, and the values obtained for scores were statistically analyzed by using the Kruskal-Wallis and Dun tests. The level of significance was set at 5%. RESULTS: ED and ED-AP groups expressed significantly higher IL-17 levels in both hepatic and renal tissues (p<0.05), compared to N and N-AP groups. Apical periodontitis (AP) in ED-AP group was significantly more severe than that in N-AP group (p<0.05). Furthermore, there was a significantly larger increase in the IL-17 levels in ED-AP group compared to N group (p<0.05). CONCLUSION: Our results indicate that diabetes increases IL-17 levels in hepatic and renal tissues and also enhances IL-17 production in apical periodontitis area of rats.