Juan Carlos Sanz1, Esther Ríos2, Iciar Rodríguez-Avial2, Belén Ramos3, Mercedes Marín4, Emilia Cercenado4. 1. Laboratorio Regional de Salud Pública de la Comunidad de Madrid, Unidad de Microbiología Clínica, Centro de Especialidades Médicas Vicente Soldevilla 2ª planta, Madrid 28053, Spain; CIBER en Epidemiología y Salud Pública (CIBERESP), Spain. Electronic address: juan.sanz@salud.madrid.org. 2. Departamento de Microbiología Clínica, Hospital Clínico San Carlos, Madrid, Spain. 3. Laboratorio Regional de Salud Pública de la Comunidad de Madrid, Unidad de Microbiología Clínica, Centro de Especialidades Médicas Vicente Soldevilla 2ª planta, Madrid 28053, Spain. 4. Servicio de Microbiología, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
Abstract
INTRODUCTION: The aim was to evaluate the utility of a multiplex real-time PCR to detect Streptococcus pneumoniae lytA, plyA and psaA genes in pleural fluid (PF). METHODS: A collection of 81 PF samples was used. Sixty were considered positive for S. pneumoniae according to previous results (54 by an in-house lytA gene PCR and eight by universal rRNA PCR). RESULTS: The sensitivity for detection of the lytA, plyA and psaA genes by multiplex PCR was 100% (60/60), 98.3% (59/60) and 91.7% (55/60), respectively. The detection of all three genes was negative in 21 samples formerly confirmed as negative for S. pneumoniae (100% specificity) by the other procedures (9 by in-house lytA PCR and 12 by rRNA PCR). CONCLUSIONS: The use of this multiplex PCR may be a useful option to identify S. pneumoniae directly in PF samples.
INTRODUCTION: The aim was to evaluate the utility of a multiplex real-time PCR to detect Streptococcus pneumoniae lytA, plyA and psaA genes in pleural fluid (PF). METHODS: A collection of 81 PF samples was used. Sixty were considered positive for S. pneumoniae according to previous results (54 by an in-house lytA gene PCR and eight by universal rRNA PCR). RESULTS: The sensitivity for detection of the lytA, plyA and psaA genes by multiplex PCR was 100% (60/60), 98.3% (59/60) and 91.7% (55/60), respectively. The detection of all three genes was negative in 21 samples formerly confirmed as negative for S. pneumoniae (100% specificity) by the other procedures (9 by in-house lytA PCR and 12 by rRNA PCR). CONCLUSIONS: The use of this multiplex PCR may be a useful option to identify S. pneumoniae directly in PF samples.
Authors: M Valerio; F López-Medrano; I Regalado-Artamendi; P Muñoz; J M Aguado; E Bouza Journal: Rev Esp Quimioter Date: 2018-06-28 Impact factor: 1.553