| Literature DB >> 28812171 |
Shaozhen Xing1, Xianglilan Zhang2, Qiang Sun2, Jian Wang2, Zhiqiang Mi2, Guangqian Pei2, Yong Huang2, Xiaoping An2, Kaifei Fu3, Lijun Zhou4, Baohua Zhao5, Yigang Tong6.
Abstract
A novel virulent bacteriophage named vB_EfaP_IME199 that specifically infects Enterococcus faecium was isolated and characterized. Its optimal multiplicity of infection was 0.01, and it had a 30 minute outbreak period. High-throughput sequencing revealed that the phage has a dsDNA genome of 18,838 bp with 22 open reading frames. The genome has very low homology to all other bacteriophage sequences in the GenBank database. Run-off sequencing experiments confirmed that vB_EfaP_IME199 has short inverted terminal repeats. Phylogenetic analysis indicated that vB_EfaP_IME199 can be taxonomically classified as a new member of the genus Ahjdlikevirus of family Podoviridae.Entities:
Mesh：
Substances：
Year: 2017 PMID： 28812171 DOI： 10.1007/s00705-017-3503-1
Source DB: PubMed Journal: Arch Virol ISSN： 0304-8608 Impact factor: 2.574