| Literature DB >> 28809022 |
Masanori Uchikawa1, Naoko Nishimura2, Makiko Iwafuchi-Doi3, Hisato Kondoh4.
Abstract
Chicken embryo electroporation is a powerful tool used to identify and analyze enhancers involved in developmental gene regulation. In this chapter, the basic procedures and underlying principles of enhancer analysis using chicken embryo electroporation are described in the following steps: (1) identification of enhancers in a wide genomic region, (2) determination of the full enhancer region, (3) definition of the core enhancer regions, and (4) analysis of a functional transcription factor binding sequences in the core region.Entities:
Keywords: Core region; Electroporation; Enhancers; Transcription factor binding; tkEGFP
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Year: 2017 PMID： 28809022 DOI： 10.1007/978-1-4939-7216-6_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745