| Literature DB >> 28808849 |
Yongle Yu1, Jun Su1, Jigui Wang1, Ji Xi1, Yaping Mao1, Qiang Hou1, Xiaomei Zhang1, Weiquan Liu2.
Abstract
Canine parvovirus (CPV) is an important and highly prevalent pathogen of dogs that causes acute hemorrhagic enteritis disease. Here, we describe a rapid method for the construction and characterization of a full-length infectious clone (rCPV) of CPV. Feline kidney (F81) cells were transfected with rCPV incorporating an engineered EcoR I site that served as a genetic marker. The rescued virus was indistinguishable from that of wild-type virus in its biological properties.Entities:
Keywords: Biological properties; Canine parvovirus; Genetic marker; Infectious clone; Rescue virus; Reverse genetics
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Year: 2017 PMID: 28808849 DOI: 10.1007/s11262-017-1497-0
Source DB: PubMed Journal: Virus Genes ISSN: 0920-8569 Impact factor: 2.332