Data on the impact of the blood sample collection methods on blood protein profiling studies.

Complete blood protein profiles of 4 different blood sample collection methods (EDTA-, heparin- and citrate plasma and serum) were investigated and the data presented herein is an extension of the research article in Ilies et al. [1]. Specimens were depleted of 6 highly abundant proteins and protein profiling was assessed by nano-LC UDMSE. Exhaustive protein sets and protein abundances before and after depletion are presented in tables and figures. Also, the core protein set and the unique proteins for each sample collection method previously described [1] are disclosed.

Specifications Table

Value of the data

Data shows a comprehensive evaluation of the different blood sample collection methods on 6 high abundant proteins and their depletion efficiency using immunoaffinity MARS6 column which can be used for future investigations on blood high abundant proteins and depleted fractions.

Individual protein abundances, their presence and variance in the samples collected with different methods after depletion are of potential value to determine which sampling method to be used for proteomics investigations.

Data presents an all-inclusive set of information on the methods applied to evaluate the impact of different blood sample collection methods on protein profiling studies and can be used as benchmark for future blood protein profiling studies.

Data

In this Data in Brief article we provide detailed information on blood protein profiling as an extension of the results reported in Ref. [1], 24 blood specimens were collected from 6 healthy and young volunteers in different sample collection tubes for serum and plasma. Tubes characteristics and the subsequent sample preparation are presented in Table 1. For the blood protein profiling a nanoLC-UDMSE method and standard search parameters were employed. Detailed description of methods can be found in Ref. [1] and its supplementary methods. 6 highly abundant blood proteins, namely serum albumin, immunoglobulin gamma, immunoglobulin alpha, serotransferrin, haptoglobin, and alpha-1-antitrypsin, were depleted by using a commercially available immunoaffinity depletion column. A detailed overview on depletion efficiency based on protein abundances for all sample collection methods is presented in Table 2. The distribution of the high abundant proteins before and after depletion is presented in Fig. 1 and more specific, fibrinogen coverage is shown in Fig. 2. Data regarding number of identified peptides and relatively quantified proteins for all sample types after depletion is shown in Fig. 3. Also, a top 10 list of the most abundant unique proteins for each of the EDTA-, heparin-, citrate plasma and serum samples is given in Table 3. The complete list of all relatively quantified proteins over all samples including their occurrence in the protein core set or as unique proteins interpreted in detail previously [1], can be found in the Supplementary material with data on individual sample abundance, mean abundance for each sample collection method and the abundance based coefficient of variation after depletion.

Table 1

Blood sample collection tubes characteristics.

Blood product	Serum	Plasma
Tube type	Plastic SST™ II Advance	Plastic K2EDTA	Glass Citrate	Glass sodium heparin
Cat. No./NHS code	367954/KFK114	367873/KFK286	367691/KFK186	367876/KFK279
Additive (concentration)	Silica (clot activator)/gel	Potassium EDTA	Buffered sodium citrate (0,105 M)	Sodium heparin (17 IU/mL blood)
Volume (mL)	5	6	4.5	6
Mixing recommendation	Gently inverted 180° and back	Gently inverted 180° and back	Gently inverted 180° and back	Gently inverted 180° and back
	5–6 times	8–10 times	3–4 times	8–10 times

Table 2

Summary of depletion efficiency.

Mean protein abundance	EDTA plasma		Heparin plasma		Citrate plasma		Serum
	Before depletion	After depletion	Before depletion	After depletion	Before depletion	After depletion	Before depletion	After depletion
All proteins	40568018.74	44741390.04	35119594.07	52949084.17	40671622.40	69872,370.90	54995897.89	45191163.47
α-1-antitrypsin	757196.34	14259.97	584561.17	23153.90	764890.57	22854.17	1006489.65	29692.67
Haptoglobin	631767.39	18029.34	487822.58	41219.67	596917.79	56161.72	689948.19	41259.85
Ig A	429327.89	44422.15	380247.33	23957.12	431941.12	51775.29	550978.46	18885.19
Ig G 1–4	2013660.86	91452.63	1677118.63	101491.82	2145431.99	168092.78	2856603.51	124679.28
Serotransferrin	2369837.17	213742.01	2096978.58	183333.16	2651548.54	364344.32	3224215.08	202618.47
Serum albumin	15235797.33	2056072.75	12925303.83	1866019.81	15394810.67	3600066.33	22315530.67	2807855.77
Other proteins	19130431.76	42303411.19	16967561.94	50709908.69	18686081.72	65609076.29	24352132.33	41966172.25
Total fibrinogen	1839721.65	7325737.50	1108301.52	6370617.46	1657777.20	9817167.88	0.00	71699.88

Fig. 1

HAP abundance before and after depletion.

Fig. 2

Total fibrinogen abundance before and after depletion of HAP.

Fig. 3

Global overview on the identified peptides and quantified proteins.

Table 3

TOP 10 abundant proteins exclusively identified per sampling method.

Accession	Entry name	TOP10_EDTA plasma Protein names	Secreted/ leakage	EDTA 1	EDTA2	EDTA3	EDTA4	EDTA5	EDTA6	Mean	CV
O75410	TACC1	Transforming acidic coiled-coil-containing protein 1	Leakage	49174	65077	109984	54229	65833	39035	63889	0.39
Q9P2D6	F135A	Protein FAM135A	Not specified	47425	51140	9049	33084	57915	46010	40770	0.43
Q99683	M3K5	Mitogen-activated protein kinase kinase kinase 5	leakage	37558	44893	34790	36856	52333	37776	40701	0.16
Q9P0W8	SPAT7	Spermatogenesis-associated protein 7	Leakage	21640	32866	28811	17938	32732	19729	25619	0.26
Q9BYW2	SETD2	Histone-lysine N-methyltransferase SETD2	Leakage	22027	26583	21030	22561	30931	20783	23986	0.17
Q15573	TAF1A	TATA box-binding protein-associated factor RNA polymerase I subunit A	Leakage	14671	20078	22199	21065	27359	18243	20603	0.21
P15813	CD1D	Antigen-presenting glycoprotein CD1d	Leakage	13395	17280	13398	14071	20923	15275	15724	0.19
O14950	ML12B	Myosin regulatory light chain 12B	Not specified	10508	9974	11784	13291	8753	9291	10600	0.16
Q5TBE3	CI153	Uncharacterized protein C9orf153	Not specified	6183	7639	8439	7543	11014	7128	7991	0.21
Q8N4P6	LRC71	Leucine-rich repeat-containing protein 71	Not specified	10463	8450	5703	7123	6758	6869	7561	0.22
Accession	Entry name	TOP10_heparin plasma Protein names	Secreted/ leakage	Heparin1	Heparin2	Heparin3	Heparin4	Heparin5	Heparin6	Mean	CV
Q8WUY3	PRUN2	Protein prune homolog 2	Leakage	160723	206288	192573	306294	247158	283487	232754	0.24
Q15811	ITSN1	Intersectin-1	Leakage	50959	63100	53081	48220	90431	34339	56688	0.33
Q00610	CLH1	Clathrin heavy chain 1	Leakage	15586	14728	18562	20815	22935	20262	18815	0.17
P24043	LAMA2	Laminin subunit alpha-2	Secreted	11392	12288	14534	17688	16610	15376	14648	0.17
Q96RE9	ZN300	Zinc finger protein 300	Leakage	7134	11365	11937	20051	15033	15472	13499	0.33
Q9P0W5	SCHI1	Schwannomin-interacting protein 1	Leakage	17022	10273	10231	14051	10710	10656	12157	0.23
Q5RL73	RBM48	RNA-binding protein 48	Not specified	5454	8424	11531	14460	8906	21118	11649	0.48
Q9P219	DAPLE	Protein Daple	Leakage	7162	10200	14709	10857	11183	10820	10822	0.22
Q8N3R3	TCAIM	T-cell activation inhibitor, mitochondrial	Leakage	13138	11101	8965	8622	9601	8057	9914	0.19
Q7LG56	RIR2B	Ribonucleoside-diphosphate reductase subunit M2 B	Leakage	5452	8109	8708	10250	8552	9235	8384	0.19

Experimental design, materials and methods

Experimental design and the materials and methods have been reported previously [1].

Subject area	Proteomics
More specific subject area	Clinical chemistry, Biomarker analysis, Blood proteome profiling
Type of data	Tables, figures (PDF file format)
How data was acquired	nano liquid chromatography (AQUITY UPLC M-CLASS, Waters Corporation) tandem mass spectrometry (Synapt G2Si mass spectrometer, WATERS Corporation)
	UDMSEdata acquisition
Data format	Analyzed and processed data
Experimental factors	24 blood samples were drawn from 6 healthy young volunteers in serum tubes and plasma tubes containing EDTA, heparin, and citrate.
Experimental features	Serum and plasma was obtained after tube manufacturer's instructions and aliquots were stored at -80  °C until analysis. Protein profiles were analyzed before and after samples depletion of 6 high abundant proteins using a commercial MARS6 (Agilent Technologies) immunoaffinity based column. Prior to the mass spectrometric analysis, proteins were digested by trypsin and peptides were further analyzed and protein profiles investigated with respect to the sample collection method influence.
Data source location	Greifswald, Germany
Data accessibility	Data is with article