| Literature DB >> 28807982 |
Akihiro Tamura1, Hideyo Hirai1, Asumi Yokota1, Naoka Kamio1, Atsushi Sato1, Tsukimi Shoji1, Takahiro Kashiwagi1, Yusuke Torikoshi1, Yasuo Miura1, Daniel G Tenen2,3, Taira Maekawa1.
Abstract
The transcription factor CCAAT/enhancer-binding protein β (C/EBPβ) is highly expressed in monocytes/macrophages. However, its roles in monopoiesis are largely unknown. Here, we investigated the roles of C/EBPβ in monopoiesis. Further subdivision of monocytes revealed that Cebpb messenger RNA was highly upregulated in Ly6C- monocytes in bone marrow. Accordingly, the number of Ly6C- monocytes was significantly reduced in Cebpb-/- mice. Bone marrow chimera experiments and Mx1-Cre-mediated deletion of Cebpb revealed a cell-intrinsic and monocyte-specific requirement for C/EBPβ in monopoiesis. In Cebpb-/- mice, turnover of Ly6C- monocytes was highly accelerated and apoptosis of Ly6C- monocytes was increased. Expression of Csf1r, which encodes a receptor for macrophage colony-stimulating factor, was significantly reduced in Ly6C- monocytes of Cebpb-/- mice. C/EBPβ bound to positive regulatory elements of Csf1r and promoted its transcription. Collectively, these results indicate that C/EBPβ is a critical factor for Ly6C- monocyte survival, at least in part through upregulation of Csf1r.Entities:
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Year: 2017 PMID： 28807982 PMCID： PMC5649551 DOI： 10.1182/blood-2017-03-772962
Source DB: PubMed Journal: Blood ISSN： 0006-4971 Impact factor: 22.113