H Yu1, H Sun2, Z Wang3, Y Liu3. 1. Department of Infectious Diseases,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,P R China. 2. Department of Otorhinolaryngology,Huazhong University of Science and Technology,Wuhan,P R China. 3. Department of Otolaryngology - Head and Neck Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,P R China.
Abstract
OBJECTIVE: To investigate the effect of microRNA let-7a on OPN expression in a mouse model of allergic rhinitis. METHODS: Thirty-two mature female C57BL/6 mice were randomly divided into four groups, with eight mice in each group: microRNA let-7a, microRNA control, OVA and phosphate-buffered saline groups. The microRNA let-7a, microRNA control and OVA groups were sensitised with OVA to establish a mouse model of allergic rhinitis. Nose-scratching events were counted. Nasal mucosa was used to evaluate histological changes of goblet cell hyperplasia. Interleukins 5 and 13 were detected. Interferon-γ levels in the nasal lavage fluid were assayed with enzyme-linked immunosorbent assay. OPN expression was estimated with polymerase chain reaction. RESULTS: Compared with microRNA control mice, microRNA let-7a treated mice had a significantly increased number of nose-scratching events, nasal mucosal eosinophilia and goblet cell hyperplasia (p 0.05). In addition, microRNA let-7a treated mice had significantly enhanced OPN expression in nasal mucosa (p < 0.05). CONCLUSION: MicroRNA let-7a can promote allergic rhinitis development partly by regulating OPN expression.
OBJECTIVE: To investigate the effect of microRNA let-7a on OPN expression in a mouse model of allergic rhinitis. METHODS: Thirty-two mature female C57BL/6 mice were randomly divided into four groups, with eight mice in each group: microRNA let-7a, microRNA control, OVA and phosphate-buffered saline groups. The microRNA let-7a, microRNA control and OVA groups were sensitised with OVA to establish a mouse model of allergic rhinitis. Nose-scratching events were counted. Nasal mucosa was used to evaluate histological changes of goblet cell hyperplasia. Interleukins 5 and 13 were detected. Interferon-γ levels in the nasal lavage fluid were assayed with enzyme-linked immunosorbent assay. OPN expression was estimated with polymerase chain reaction. RESULTS: Compared with microRNA control mice, microRNA let-7a treated mice had a significantly increased number of nose-scratching events, nasal mucosal eosinophilia and goblet cell hyperplasia (p 0.05). In addition, microRNA let-7a treated mice had significantly enhanced OPN expression in nasal mucosa (p < 0.05). CONCLUSION: MicroRNA let-7a can promote allergic rhinitis development partly by regulating OPN expression.
Authors: Susrutha Puthanmadhom Narayanan; Daniel R O'Brien; Mayank Sharma; Thomas C Smyrk; Rondell P Graham; Madhusudan Grover; Adil E Bharucha Journal: Clin Gastroenterol Hepatol Date: 2021-10-02 Impact factor: 13.576