Literature DB >> 28806851

Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique.

Adrian G Cadar1,2, Tromondae K Feaster3, Matthew D Durbin4, Charles C Hong2,5.   

Abstract

This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications. © 2017 by John Wiley & Sons, Inc.
Copyright © 2017 John Wiley and Sons, Inc.

Entities:  

Keywords:  Matrigel; cardiac; hiPSC-CMs; myocytes; stem cells

Mesh:

Substances:

Year:  2017        PMID: 28806851      PMCID: PMC5577013          DOI: 10.1002/cpsc.32

Source DB:  PubMed          Journal:  Curr Protoc Stem Cell Biol        ISSN: 1938-8969


  14 in total

1.  Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness.

Authors:  Alexandre J S Ribeiro; Yen-Sin Ang; Ji-Dong Fu; Renee N Rivas; Tamer M A Mohamed; Gadryn C Higgs; Deepak Srivastava; Beth L Pruitt
Journal:  Proc Natl Acad Sci U S A       Date:  2015-09-28       Impact factor: 11.205

2.  Measuring the contractile forces of human induced pluripotent stem cell-derived cardiomyocytes with arrays of microposts.

Authors:  Marita L Rodriguez; Brandon T Graham; Lil M Pabon; Sangyoon J Han; Charles E Murry; Nathan J Sniadecki
Journal:  J Biomech Eng       Date:  2014-05       Impact factor: 2.097

Review 3.  Differentiation of human embryonic stem cells and induced pluripotent stem cells to cardiomyocytes: a methods overview.

Authors:  Christine L Mummery; Jianhua Zhang; Elizabeth S Ng; David A Elliott; Andrew G Elefanty; Timothy J Kamp
Journal:  Circ Res       Date:  2012-07-20       Impact factor: 17.367

4.  Forecasting the impact of heart failure in the United States: a policy statement from the American Heart Association.

Authors:  Paul A Heidenreich; Nancy M Albert; Larry A Allen; David A Bluemke; Javed Butler; Gregg C Fonarow; John S Ikonomidis; Olga Khavjou; Marvin A Konstam; Thomas M Maddox; Graham Nichol; Michael Pham; Ileana L Piña; Justin G Trogdon
Journal:  Circ Heart Fail       Date:  2013-04-24       Impact factor: 8.790

5.  The elastic modulus of Matrigel as determined by atomic force microscopy.

Authors:  Shauheen S Soofi; Julie A Last; Sara J Liliensiek; Paul F Nealey; Christopher J Murphy
Journal:  J Struct Biol       Date:  2009-05-27       Impact factor: 2.867

6.  Identification of multiple active growth factors in basement membrane Matrigel suggests caution in interpretation of cellular activity related to extracellular matrix components.

Authors:  S Vukicevic; H K Kleinman; F P Luyten; A B Roberts; N S Roche; A H Reddi
Journal:  Exp Cell Res       Date:  1992-09       Impact factor: 3.905

7.  Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980-2015: a systematic analysis for the Global Burden of Disease Study 2015.

Authors: 
Journal:  Lancet       Date:  2016-10-08       Impact factor: 79.321

8.  Atomic force mechanobiology of pluripotent stem cell-derived cardiomyocytes.

Authors:  Jianwei Liu; Ning Sun; Marc A Bruce; Joseph C Wu; Manish J Butte
Journal:  PLoS One       Date:  2012-05-18       Impact factor: 3.240

9.  HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.

Authors:  John T Hinson; Anant Chopra; Navid Nafissi; William J Polacheck; Craig C Benson; Sandra Swist; Joshua Gorham; Luhan Yang; Sebastian Schafer; Calvin C Sheng; Alireza Haghighi; Jason Homsy; Norbert Hubner; George Church; Stuart A Cook; Wolfgang A Linke; Christopher S Chen; J G Seidman; Christine E Seidman
Journal:  Science       Date:  2015-08-28       Impact factor: 47.728

10.  Video image-based analysis of single human induced pluripotent stem cell derived cardiomyocyte beating dynamics using digital image correlation.

Authors:  Antti Ahola; Anna L Kiviaho; Kim Larsson; Markus Honkanen; Katriina Aalto-Setälä; Jari Hyttinen
Journal:  Biomed Eng Online       Date:  2014-04-07       Impact factor: 2.819

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  3 in total

1.  Real-time visualization of titin dynamics reveals extensive reversible photobleaching in human induced pluripotent stem cell-derived cardiomyocytes.

Authors:  Adrian G Cadar; Tromondae K Feaster; Kevin R Bersell; Lili Wang; TingTing Hong; Joseph A Balsamo; Zhentao Zhang; Young Wook Chun; Young-Jae Nam; Michael Gotthardt; Björn C Knollmann; Dan M Roden; Chee C Lim; Charles C Hong
Journal:  Am J Physiol Cell Physiol       Date:  2019-11-20       Impact factor: 4.249

Review 2.  Biodegradable Nanopolymers in Cardiac Tissue Engineering: From Concept Towards Nanomedicine.

Authors:  Saeed Mohammadi Nasr; Navid Rabiee; Sakineh Hajebi; Sepideh Ahmadi; Yousef Fatahi; Masoumehossadat Hosseini; Mojtaba Bagherzadeh; Amir Mohammad Ghadiri; Mohammad Rabiee; Vahid Jajarmi; Thomas J Webster
Journal:  Int J Nanomedicine       Date:  2020-06-18

3.  End-to-End Platform for Human Pluripotent Stem Cell Manufacturing.

Authors:  Puspa R Pandey; Amarel Tomney; Marites T Woon; Nicholas Uth; Farjad Shafighi; Igor Ngabo; Haritha Vallabhaneni; Yonatan Levinson; Eytan Abraham; Inbar Friedrich Ben-Nun
Journal:  Int J Mol Sci       Date:  2019-12-21       Impact factor: 5.923

  3 in total

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