| Literature DB >> 28806851 |
Adrian G Cadar1,2, Tromondae K Feaster3, Matthew D Durbin4, Charles C Hong2,5.
Abstract
This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications. © 2017 by John Wiley & Sons, Inc.Entities:
Keywords: Matrigel; cardiac; hiPSC-CMs; myocytes; stem cells
Mesh:
Substances:
Year: 2017 PMID: 28806851 PMCID: PMC5577013 DOI: 10.1002/cpsc.32
Source DB: PubMed Journal: Curr Protoc Stem Cell Biol ISSN: 1938-8969