Literature DB >> 28804842

Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood.

Leonardo Pasalic1,2, Gabrielle J Pennings3, David Connor4, Heather Campbell5, Leonard Kritharides3,6, Vivien M Chen3,5,7.   

Abstract

Flow cytometry is a powerful tool for rapid evaluation of multiple functional properties of large numbers of platelets in whole blood. In the following chapter, we provide a number of flow cytometry-based protocols broadly aimed at (1) assessment of constitutively expressed platelet membrane receptors to diagnose inherited platelet bleeding disorders and (2) investigation of basal and agonist-induced platelet functional responses including generation of platelet-leukocyte aggregates, alpha and dense granule release, calcium flux, and phosphatidylserine exposure.

Entities:  

Keywords:  Calcium flux; Dense granule release; Flow cytometry; Glycoprotein receptor deficiency; Platelet function; Platelet-leukocyte aggregates; Procoagulant platelets; Whole blood

Mesh：

Substances：

Year:  2017        PMID： 28804842     DOI： 10.1007/978-1-4939-7196-1_28

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Procoagulant platelets: Generation, characteristics, and therapeutic target.

Authors:  Yaxin Chu; Han Guo; Yuncong Zhang; Rui Qiao
Journal:  J Clin Lab Anal       Date:  2021-03-11       Impact factor: 2.352

2.  Novel assay demonstrates that coronary artery disease patients have heightened procoagulant platelet response.

Authors:  L Pasalic; E Wing-Lun; J K Lau; H Campbell; G J Pennings; E Lau; D Connor; H P Liang; D Muller; L Kritharides; P J Hogg; V M Chen
Journal:  J Thromb Haemost       Date:  2018-05-08       Impact factor: 5.824

Review 3.  ROS in Platelet Biology: Functional Aspects and Methodological Insights.

Authors:  Elena Masselli; Giulia Pozzi; Mauro Vaccarezza; Prisco Mirandola; Daniela Galli; Marco Vitale; Cecilia Carubbi; Giuliana Gobbi
Journal:  Int J Mol Sci       Date:  2020-07-09       Impact factor: 5.923

4.  Cilostazol combined with P2Y12 receptor inhibitors: A substitute antiplatelet regimen for aspirin-intolerant patients undergoing percutaneous coronary stent implantation.

Authors:  Yikai Zhao; Peng Zhou; Wen Gao; Haoxuan Zhong; Yufei Chen; Wei Chen; Maieryemu Waresi; Kun Xie; Haiming Shi; Hui Gong; Guibin He; Zhaohui Qiu; Xinping Luo; Jian Li
Journal:  Clin Cardiol       Date:  2022-02-04       Impact factor: 2.882

5.  Colchicine inhibits ROS generation in response to glycoprotein VI stimulation.

Authors:  G J Pennings; C J Reddel; M Traini; H Campbell; V Chen; L Kritharides
Journal:  Sci Rep       Date:  2021-06-07       Impact factor: 4.379
  5 in total

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