Baoru Liu1,2, Yueping Luo1,2, Dong Luo1,2, Weichen Zhou1,2, Yu Zhang1,2, Ruixin He1,2, Junshu Li1,2, Yong Wang1,2, Yan Wang1,2, Wenzhi Chen2,3. 1. State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and The Ministry of Science and Technology, Chongqing Key Laboratory of Biomedical Engineering, College of Biomedical Engineering, Chongqing Medical UniversityChongqing 400016, China. 2. Chongqing Collaborative Innovation Center for Minimally-Invasive and Noninvasive MedicineChongqing 400016, China. 3. Clinical Center for Tumor Therapy, The 2nd Affiliated Hospital of Chongqing Medical UniversityChongqing 400010, China.
Abstract
OBJECTIVE: This study aims to examine the effects of low intensity pulsed ultrasound (LIPUS) on leukopenia induced by cyclophosphamide in a rabbit model. METHODS: The leukopenia model in New Zealand rabbit was established by injecting cyclophosphamide into the ear vein. Forty leukopenia model rabbits were randomly allocated to control group (n = 20) and LIPUS group (n = 20). LIPUS group underwent 20 minutes of daily ultrasound treatment at femoral metaphysis for 7 days while control group received sham treatment. Diarrhea rate, mortality and blood cell count were calculated. IgA, IgG and IgM levels were measured by ELISA. Flow cytometry was used to detect CD44, CD49d, and PU.1. HE staining was performed to analyze bone marrow hyperplasia and changes of skin and muscle. RESULTS: LIPUS treatment significantly promoted the proliferation of bone marrow nucleated cells, increased the number of WBC, IgA, IgG and IgM in the peripheral blood, and reduced the diarrhea rate and mortality. The irradiated skin and muscle tissues showed no obvious damages. LIPUS treatment promoted the migration of hematopoietic cells to peripheral blood by decreasing the expression of CD49d and CD44 on the surface of CD34 positive cells. It also promoted the differentiation of hematopoietic stem cells into granulocytes and lymphocytes by decreasing the expression of PU.1. CONCLUSION: LIPUS can be used as a safe and effective clinical treatment for cyclophosphamide induced leukopenia.
OBJECTIVE: This study aims to examine the effects of low intensity pulsed ultrasound (LIPUS) on leukopenia induced by cyclophosphamide in a rabbit model. METHODS: The leukopenia model in New Zealand rabbit was established by injecting cyclophosphamide into the ear vein. Forty leukopenia model rabbits were randomly allocated to control group (n = 20) and LIPUS group (n = 20). LIPUS group underwent 20 minutes of daily ultrasound treatment at femoral metaphysis for 7 days while control group received sham treatment. Diarrhea rate, mortality and blood cell count were calculated. IgA, IgG and IgM levels were measured by ELISA. Flow cytometry was used to detect CD44, CD49d, and PU.1. HE staining was performed to analyze bone marrow hyperplasia and changes of skin and muscle. RESULTS: LIPUS treatment significantly promoted the proliferation of bone marrow nucleated cells, increased the number of WBC, IgA, IgG and IgM in the peripheral blood, and reduced the diarrhea rate and mortality. The irradiated skin and muscle tissues showed no obvious damages. LIPUS treatment promoted the migration of hematopoietic cells to peripheral blood by decreasing the expression of CD49d and CD44 on the surface of CD34 positive cells. It also promoted the differentiation of hematopoietic stem cells into granulocytes and lymphocytes by decreasing the expression of PU.1. CONCLUSION: LIPUS can be used as a safe and effective clinical treatment for cyclophosphamide induced leukopenia.
Authors: C M Korstjens; R H H van der Rijt; G H R Albers; C M Semeins; J Klein-Nulend Journal: Med Biol Eng Comput Date: 2008-10-14 Impact factor: 2.602
Authors: Dong Luo; Yue-Ping Luo; Bao-Ru Liu; Dan-Dan Liang; Jing-Wei Jiang; Wei Wang; Jun-Lin Chen; Yan Wang; Wen-Zhi Chen Journal: Nan Fang Yi Ke Da Xue Xue Bao Date: 2017-12-20