| Literature DB >> 28803543 |
Alexey A Pakhomov1, Igor E Deyev1, Natalia M Ratnikova1, Stepan P Chumakov1, Veronika B Mironiuk1,2, Yuriy N Kononevich2, Aziz M Muzafarov2,3, Vladimir I Martynov1.
Abstract
In nonpolar solvents, hydrophobic organic fluorophores often show bright fluorescence, whereas in polar media, they usually suffer from aggregation-caused quenching (ACQ). Here, we harnessed this solvatochromic behavior of a 1,3,5,7-tetramethyl-BODIPY derivative for cell staining and applied it to live-cell imaging and flow cytometry. As opposed to commercially available dyes, this BODIPY derivative showed excellent contrast immediately after staining and did not require any wash-off.Entities:
Keywords: BODIPY; aggregation-caused quenching; flow cytometry; fluorescence microscopy; fluorophore; live-cell imaging
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Year: 2017 PMID： 28803543 DOI： 10.2144/000114577
Source DB: PubMed Journal: Biotechniques ISSN： 0736-6205 Impact factor: 1.993