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Literature DB >> 28802722

Quantification of biofilm biomass by staining: Non-toxic safranin can replace the popular crystal violet.

Pernille Ommen¹, Natalia Zobek¹, Rikke Louise Meyer².

Abstract

Crystal violet staining is commonly used for quantification of biofilm formation, although it is highly toxic. Here we test safranin as a non-toxic replacement. Safranin staining provided similar results as crystal violet, but with higher reproducibility. We therefore recommend safranin staining for biofilm biomass quantification.

Entities: Chemical

Keywords: Biofilm quantification; CV assay; Crystal violet; Safranin

Mesh：

Substances：

Year: 2017 PMID： 28802722 DOI： 10.1016/j.mimet.2017.08.003

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

14 in total

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Review 2. Microtiter plate assays to assess antibiofilm activity against bacteria.

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3. Antimicrobial Effects of _L-Chg₁₀-Teixobactin against Enterococcus faecalis In Vitro.

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Journal: Microorganisms Date: 2022-05-26

4. Study of the effects of mineral salts on the biofilm formation on polypropylene fibers using three quantification methods.

Authors: Lukáš Bystrianský; Martina Hujslová; Milan Gryndler
Journal: Folia Microbiol (Praha) Date: 2020-10-26 Impact factor: 2.099

5. Assessing biofilm inhibition and immunomodulatory activity of small amounts of synthetic host defense peptides synthesized using SPOT-array technology.

Authors: Hashem Etayash; Evan F Haney; Robert E W Hancock
Journal: Nat Protoc Date: 2021-04-09 Impact factor: 13.491

Quantification of biofilm biomass by staining: Non-toxic safranin can replace the popular crystal violet.

1. Xenorhabdus bovienii strain jolietti uses a type 6 secretion system to kill closely related Xenorhabdus strains.

Review 2. Microtiter plate assays to assess antibiofilm activity against bacteria.

3. Antimicrobial Effects of _L-Chg₁₀-Teixobactin against Enterococcus faecalis In Vitro.

4. Study of the effects of mineral salts on the biofilm formation on polypropylene fibers using three quantification methods.

5. Assessing biofilm inhibition and immunomodulatory activity of small amounts of synthetic host defense peptides synthesized using SPOT-array technology.

6. Critical Assessment of Methods to Quantify Biofilm Growth and Evaluate Antibiofilm Activity of Host Defence Peptides.

7. Influence of Probiotic Culture Supernatants on In Vitro Biofilm Formation of Staphylococci.

Review 8. Methods Used for the Eradication of Staphylococcal Biofilms.

Review 9. Microbial Biofilms in the Food Industry-A Comprehensive Review.

Review 10. Current Status of In Vitro Models and Assays for Susceptibility Testing for Wound Biofilm Infections.

Quantification of biofilm biomass by staining: Non-toxic safranin can replace the popular crystal violet.

1. Xenorhabdus bovienii strain jolietti uses a type 6 secretion system to kill closely related Xenorhabdus strains.

Review 2. Microtiter plate assays to assess antibiofilm activity against bacteria.

3. Antimicrobial Effects of L-Chg10-Teixobactin against Enterococcus faecalis In Vitro.

4. Study of the effects of mineral salts on the biofilm formation on polypropylene fibers using three quantification methods.

5. Assessing biofilm inhibition and immunomodulatory activity of small amounts of synthetic host defense peptides synthesized using SPOT-array technology.

6. Critical Assessment of Methods to Quantify Biofilm Growth and Evaluate Antibiofilm Activity of Host Defence Peptides.

7. Influence of Probiotic Culture Supernatants on In Vitro Biofilm Formation of Staphylococci.

Review 8. Methods Used for the Eradication of Staphylococcal Biofilms.

Review 9. Microbial Biofilms in the Food Industry-A Comprehensive Review.

Review 10. Current Status of In Vitro Models and Assays for Susceptibility Testing for Wound Biofilm Infections.

3. Antimicrobial Effects of _L-Chg₁₀-Teixobactin against Enterococcus faecalis In Vitro.