Suoning Liu1, Jian Suo1, Chunxi Wang2, Xuan Sun1, Daguang Wang1, Liang He1, Yang Zhang1, Wei Li1. 1. Department of the Gastrointestinal Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, China. 2. Department of Urology, The First Hospital of Jilin University, Changchun, Jilin 130021, China.
Abstract
BACKGROUND: An overwhelming amount of evidence has emerged suggesting that dysregulated microRNAs (miRNAs) play crucial roles in tumorigenesis. OBJECTIVE: The study was to analyze tissue/serum miR-144 expression in gastric cancer and then evaluate their potential to predict the prognosis of gastric cancer. METHODS: We examined miR-144 levels in tissues and peripheral blood samples from 96 gastric cancer patients using real-time PCR. Then the association between tissue/serum miR-144 levels and clinicopathological parameters was determined. RESULTS: The expression levels of miR-144 were significantly down-regulated in the cancerous tissue and serum samples from gastric cancer patients. Serum miR-144 was able to differentiate the gastric cancer patients from healthy controls with high accuracy. In addition, tissue and serum miR-144 levels were both associated with clinical stage and lymph node metastasis. Moreover, patients with lower tissue or serum miR-144 suffered worse 5 year overall survival and disease free survival. CONCLUSIONS: Taken together, our data support the potential clinical value of tissue and serum miR-144 as prognostic biomarkers in gastric cancer.
BACKGROUND: An overwhelming amount of evidence has emerged suggesting that dysregulated microRNAs (miRNAs) play crucial roles in tumorigenesis. OBJECTIVE: The study was to analyze tissue/serum miR-144 expression in gastric cancer and then evaluate their potential to predict the prognosis of gastric cancer. METHODS: We examined miR-144 levels in tissues and peripheral blood samples from 96 gastric cancerpatients using real-time PCR. Then the association between tissue/serum miR-144 levels and clinicopathological parameters was determined. RESULTS: The expression levels of miR-144 were significantly down-regulated in the cancerous tissue and serum samples from gastric cancerpatients. Serum miR-144 was able to differentiate the gastric cancerpatients from healthy controls with high accuracy. In addition, tissue and serum miR-144 levels were both associated with clinical stage and lymph node metastasis. Moreover, patients with lower tissue or serum miR-144 suffered worse 5 year overall survival and disease free survival. CONCLUSIONS: Taken together, our data support the potential clinical value of tissue and serum miR-144 as prognostic biomarkers in gastric cancer.