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Literature DB >> 2879537

Proton-translocating adenosine triphosphatase of chromaffin-granule membranes. The active site is in the largest (70 kDa) subunit.

Abstract

The proton-translocating adenosine triphosphatase (ATPase) of bovine chromaffin granules contains up to five different polypeptides. Its activity is inhibited by N-ethylmaleimide, and ATP protects the enzyme from inhibition. After treatment of membranes with N-[2-3H]ethylmaleimide, only one polypeptide is strongly radiolabelled: this is the largest (70 kDa) subunit of the proton-translocating ATPase. This subunit therefore contains the ATP-hydrolysing site. Two-dimensional electrophoresis reveals heterogeneity in this polypeptide.

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Year: 1986 PMID： 2879537 PMCID： PMC1147241 DOI： 10.1042/bj2390077

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

22 in total

1. Partial purification of a tonoplast ATPase from corn coleoptiles.

Authors: S Mandala; L Taiz
Journal: Plant Physiol Date: 1985-06 Impact factor: 8.340

2. Structural studies of the vacuolar membrane ATPase from Neurospora crassa and comparison with the tonoplast membrane ATPase from Zea mays.

Authors: E J Bowman; S Mandala; L Taiz; B J Bowman
Journal: Proc Natl Acad Sci U S A Date: 1986-01 Impact factor: 11.205

3. Fractionation of membrane proteins by temperature-induced phase separation in Triton X-114. Application to subcellular fractions of the adrenal medulla.

Authors: J G Pryde; J H Phillips
Journal: Biochem J Date: 1986-01-15 Impact factor: 3.857

4. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

Authors: J P Chamberlain
Journal: Anal Biochem Date: 1979-09-15 Impact factor: 3.365

5. Primary structure and subunit stoichiometry of F1-ATPase from bovine mitochondria.

Authors: J E Walker; I M Fearnley; N J Gay; B W Gibson; F D Northrop; S J Powell; M J Runswick; M Saraste; V L Tybulewicz
Journal: J Mol Biol Date: 1985-08-20 Impact factor: 5.469

6. Phase separation of integral membrane proteins in Triton X-114 solution.

Authors: C Bordier
Journal: J Biol Chem Date: 1981-02-25 Impact factor: 5.157

7. The molecular organization of adrenal chromaffin granules.

Authors: H Winkler; E Westhead
Journal: Neuroscience Date: 1980 Impact factor: 3.590

8. Isolation of ATPase I, the proton pump of chromaffin-granule membranes.

Authors: J M Percy; J G Pryde; D K Apps
Journal: Biochem J Date: 1985-11-01 Impact factor: 3.857

9. Properties of the partially purified tonoplast H+-pumping ATPase from oat roots.

Authors: S K Randall; H Sze
Journal: J Biol Chem Date: 1986-01-25 Impact factor: 5.157

10. Identification of 3-O-(4-benzoyl)benzoyladenosine 5'-triphosphate- and N,N'-dicyclohexylcarbodiimide-binding subunits of a higher plant H+-translocating tonoplast ATPase.

Authors: M F Manolson; P A Rea; R J Poole
Journal: J Biol Chem Date: 1985-10-05 Impact factor: 5.157

9 in total

4. Identification and characterization of glycoproteins after extraction of bovine chromaffin-granule membranes with lithium di-iodosalicylate. Purification of glycoprotein II from the soluble fraction.

Authors: D L Christie; D J Palmer
Journal: Biochem J Date: 1990-08-15 Impact factor: 3.857

9. Effects of the sulfhydryl reagent N-ethylmaleimide on reserpine binding to the catecholamine transporter in chromaffin granule membranes.

Authors: J D Deupree; J J Hitchcock
Journal: Cell Mol Neurobiol Date: 1988-06 Impact factor: 5.046

9 in total

Proton-translocating adenosine triphosphatase of chromaffin-granule membranes. The active site is in the largest (70 kDa) subunit.

1. Partial purification of a tonoplast ATPase from corn coleoptiles.

2. Structural studies of the vacuolar membrane ATPase from Neurospora crassa and comparison with the tonoplast membrane ATPase from Zea mays.

3. Fractionation of membrane proteins by temperature-induced phase separation in Triton X-114. Application to subcellular fractions of the adrenal medulla.

4. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

5. Primary structure and subunit stoichiometry of F1-ATPase from bovine mitochondria.

6. Phase separation of integral membrane proteins in Triton X-114 solution.

7. The molecular organization of adrenal chromaffin granules.

8. Isolation of ATPase I, the proton pump of chromaffin-granule membranes.

9. Properties of the partially purified tonoplast H+-pumping ATPase from oat roots.

10. Identification of 3-O-(4-benzoyl)benzoyladenosine 5'-triphosphate- and N,N'-dicyclohexylcarbodiimide-binding subunits of a higher plant H+-translocating tonoplast ATPase.

Review 1. Structural conservation and functional diversity of V-ATPases.

Review 2. Vacuolar H(+)-ATPase: from mammals to yeast and back.

Review 3. Structure, molecular genetics, and evolution of vacuolar H+-ATPases.

4. Identification and characterization of glycoproteins after extraction of bovine chromaffin-granule membranes with lithium di-iodosalicylate. Purification of glycoprotein II from the soluble fraction.

5. Immunocytochemical Localization of the Vacuolar H-ATPase in Maize Root Tip Cells.

6. Topography of a vacuolar-type H+-translocating ATPase: chromaffin-granule membrane ATPase I.

7. A sodium/proton antiporter in chromaffin-granule membranes.

8. Characterization of Mg2+-ATPase activity in isolated B16 murine melanoma melanosomes.

9. Effects of the sulfhydryl reagent N-ethylmaleimide on reserpine binding to the catecholamine transporter in chromaffin granule membranes.