Yao Chen1,2,3,4, Ling Wang1,2,3,4, Hao Liu1,2,3,4, Fahuan Song1,2,3,4, Caiyun Xu1,2,3,4, Kai Zhang1,2,3,4, Qing Chen1,2,3,4, Shuang Wu1,2,3,4, Yunqi Zhu1,2,3,4, Ying Dong5, Min Zhou1,2,3,4,6, Hong Zhang1,2,3,4, Mei Tian7,8,9,10. 1. Department of Nuclear Medicine, The Second Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang, 310009, China. 2. Zhejiang University Medical PET Center, Zhejiang University, Hangzhou, China. 3. Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China. 4. Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China. 5. Department of Oncology, The Second Hospital of Zhejiang University School of Medicine, Hangzhou, China. 6. Institute of Translational Medicine, Zhejiang University, Hangzhou, China. 7. Department of Nuclear Medicine, The Second Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou, Zhejiang, 310009, China. meitian@zju.edu.cn. 8. Zhejiang University Medical PET Center, Zhejiang University, Hangzhou, China. meitian@zju.edu.cn. 9. Institute of Nuclear Medicine and Molecular Imaging, Zhejiang University, Hangzhou, China. meitian@zju.edu.cn. 10. Key Laboratory of Medical Molecular Imaging of Zhejiang Province, Hangzhou, China. meitian@zju.edu.cn.
Abstract
PURPOSE: The aim of the study was to non-invasively evaluate the anticancer activity of a traditional Chinese medicine-Huaier, combined with paclitaxel (PTX) in breast cancer bearing mice by detecting dynamic metabolic changes with positron emission tomography (PET). PROCEDURES: Balb/c nude mice were randomly divided into one of the four groups: Huaier, PTX, PTX + Huaier, or the control. PET imaging with 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) was performed to monitor the metabolic changes in BT474 (luminal B) and MDA-MB-231 (triple-negative) breast cancer xenografts. Immunohistochemistry (IHC) study was performed immediately after the final PET scan to assess the expressions of phosphatidylinositol 3-kinase (PI3K), phospho-AKT (p-AKT), caspase-3, and vascular endothelial growth factor (VEGF). RESULTS: Compared to the control group, [18F]FDG accumulation demonstrated a significant decrease in PTX + Huaier (p < 0.01) or Huaier group (p < 0.05), which was consistent to the decreased expression of PI3K (p < 0.05) and p-AKT (p < 0.05) in the breast cancer xenografts. CONCLUSION: The therapeutic effect of Huaier combined with PTX was superior than the PTX alone in BT474 and MDA-MB-231 breast cancer-bearing mice. [18F]FDG PET imaging could be a potential non-invasive approach to assess the metabolic changes after chemotherapy combined with traditional Chinese medicine in the breast cancer.
PURPOSE: The aim of the study was to non-invasively evaluate the anticancer activity of a traditional Chinese medicine-Huaier, combined with paclitaxel (PTX) in breast cancer bearing mice by detecting dynamic metabolic changes with positron emission tomography (PET). PROCEDURES: Balb/c nude mice were randomly divided into one of the four groups: Huaier, PTX, PTX + Huaier, or the control. PET imaging with 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG) was performed to monitor the metabolic changes in BT474 (luminal B) and MDA-MB-231 (triple-negative) breast cancer xenografts. Immunohistochemistry (IHC) study was performed immediately after the final PET scan to assess the expressions of phosphatidylinositol 3-kinase (PI3K), phospho-AKT (p-AKT), caspase-3, and vascular endothelial growth factor (VEGF). RESULTS: Compared to the control group, [18F]FDG accumulation demonstrated a significant decrease in PTX + Huaier (p < 0.01) or Huaier group (p < 0.05), which was consistent to the decreased expression of PI3K (p < 0.05) and p-AKT (p < 0.05) in the breast cancer xenografts. CONCLUSION: The therapeutic effect of Huaier combined with PTX was superior than the PTX alone in BT474 and MDA-MB-231 breast cancer-bearing mice. [18F]FDG PET imaging could be a potential non-invasive approach to assess the metabolic changes after chemotherapy combined with traditional Chinese medicine in the breast cancer.
Entities:
Keywords:
Breast cancer; Paclitaxel; Positron emission tomography (PET); Traditional Chinese medicine (TCM)
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