Roser Guiteras1, Anna Sola1, Maria Flaquer1, Georgina Hotter2, Joan Torras1,3, Josep Maria Grinyó1,3, Josep Maria Cruzado1,3. 1. Experimental Nephrology, Department of Ciències Clíniques, Universitat de Barcelona, Institut d'Investigació biomèdica de Bellvitge (IDIBELL), Hospitalet de Llobregat, Barcelona, Spain. 2. Department of Ischemia and Inflammation, Institut d'lnvestigacions Biomèdiques de Barcelona (IIBB), Barcelona, Spain. 3. Hospital Universitari de Bellvitge, Hospitalet de Llobregat, Barcelona, Spain.
Abstract
BACKGROUND/AIMS: Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation and fibrosis in the unilateral ureteral obstruction (UUO) mice model. METHODS: We stabilized macrophages by adenoviral vector NGAL (Neutrophil gelatinase-associated lipocalin-2) and infused them into UUO mice. To ascertain whether macrophages were capable of reaching the obstructed kidney, macrophages were stained and detected by in vivo cell tracking. RESULTS: We demonstrated that some infused macrophages reached the obstructed kidney and that infusion of macrophages overexpressing NGAL was associated with reduced kidney interstitial fibrosis and inflammation. This therapeutic effect was mainly associated with the phenotype and function preservation of the transferred macrophages isolated from the obstructed kidney Conclusions: Macrophage plasticity is a major hurdle for achieving macrophage therapy success in chronic nephropathies and could be overcome by transferring lipocalin-2.
BACKGROUND/AIMS: Alternatively activated macrophages (AAM) have regenerative and anti-inflammatory characteristics. Here, we sought to evaluate whether AAM cell therapy reduces renal inflammation and fibrosis in the unilateral ureteral obstruction (UUO) mice model. METHODS: We stabilized macrophages by adenoviral vector NGAL (Neutrophil gelatinase-associated lipocalin-2) and infused them into UUO mice. To ascertain whether macrophages were capable of reaching the obstructed kidney, macrophages were stained and detected by in vivo cell tracking. RESULTS: We demonstrated that some infused macrophages reached the obstructed kidney and that infusion of macrophages overexpressing NGAL was associated with reduced kidney interstitial fibrosis and inflammation. This therapeutic effect was mainly associated with the phenotype and function preservation of the transferred macrophages isolated from the obstructed kidney Conclusions: Macrophage plasticity is a major hurdle for achieving macrophage therapy success in chronic nephropathies and could be overcome by transferring lipocalin-2.
Authors: Alonso Narváez; Roser Guiteras; Anna Sola; Anna Manonelles; Juan Morote; Juan Torras; Josep M Grinyó; Josep M Cruzado Journal: PLoS One Date: 2019-04-12 Impact factor: 3.240
Authors: Roser Guiteras; Anna Sola; Maria Flaquer; Anna Manonelles; Georgina Hotter; Josep M Cruzado Journal: J Cell Mol Med Date: 2018-11-08 Impact factor: 5.310
Authors: Johannes Nordlohne; Ilona Hulsmann; Svenja Schwafertz; Jasmin Zgrajek; Manuel Grundmann; Sibylle von Vietinghoff; Frank Eitner; Michael S Becker Journal: Sci Rep Date: 2021-06-24 Impact factor: 4.379