BACKGROUND: Soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) is a cell-surface receptor presented on neutrophils, macrophages and monocytes. Elevated sTREM-1 levels are a marker of acute microbial infections, and have also been reported in chronic lung diseases. IL-6 and IL-13 are effective markers in distinguishing these diseases. IL-6 has been shown to play an important role in chronic obstructive pulmonary disease (COPD), while IL-13 is described as one of the key mediators of allergic asthma. OBJECTIVES: The aim of the study was to evaluate the level of sTREM-1 in bronchoalveolar lavage fluid (BALF) from stable mild-to-moderate asthma and COPD patients and to compare the utility of BALF sTREM-1 measurements in asthma/COPD differentiation with those of IL-6 and IL-13. MATERIAL AND METHODS: The concentration of sTREM-1, IL-6 and IL-13 was evaluated by ELISA in the BALF of stable mild-to-moderate asthma and COPD patients. RESULTS: There were no significant differences in BALF sTREM-1 levels between asthma and COPD patients (52.5 pg/mL for asthma vs 73.4 pg/mL for COPD, p = 0.492), in contrast to the differences in the IL6/IL13 ratio (0.68 in COPD, 0.22 in asthma, p = 0.043). CONCLUSIONS: The study showed that BALF sTREM-1 levels do not discriminate between mild-to-moderate asthma and COPD. In contrast, the IL-6/IL-13 ratio measured in BALF effectively differentiated these two diseases in their stable state. These results suggest that the most important marker of inflammation in mild-to-moderate obstructive lung disease is not microbiology-dependent.
BACKGROUND: Soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) is a cell-surface receptor presented on neutrophils, macrophages and monocytes. Elevated sTREM-1 levels are a marker of acute microbial infections, and have also been reported in chronic lung diseases. IL-6 and IL-13 are effective markers in distinguishing these diseases. IL-6 has been shown to play an important role in chronic obstructive pulmonary disease (COPD), while IL-13 is described as one of the key mediators of allergic asthma. OBJECTIVES: The aim of the study was to evaluate the level of sTREM-1 in bronchoalveolar lavage fluid (BALF) from stable mild-to-moderate asthma and COPDpatients and to compare the utility of BALF sTREM-1 measurements in asthma/COPD differentiation with those of IL-6 and IL-13. MATERIAL AND METHODS: The concentration of sTREM-1, IL-6 and IL-13 was evaluated by ELISA in the BALF of stable mild-to-moderate asthma and COPDpatients. RESULTS: There were no significant differences in BALF sTREM-1 levels between asthma and COPDpatients (52.5 pg/mL for asthma vs 73.4 pg/mL for COPD, p = 0.492), in contrast to the differences in the IL6/IL13 ratio (0.68 in COPD, 0.22 in asthma, p = 0.043). CONCLUSIONS: The study showed that BALF sTREM-1 levels do not discriminate between mild-to-moderate asthma and COPD. In contrast, the IL-6/IL-13 ratio measured in BALF effectively differentiated these two diseases in their stable state. These results suggest that the most important marker of inflammation in mild-to-moderate obstructive lung disease is not microbiology-dependent.
Authors: Enrique Ambrocio-Ortiz; Gustavo Galicia-Negrete; Gloria Pérez-Rubio; Areli J Escobar-Morales; Edgar Abarca-Rojano; Alma D Del Angel-Pablo; Manuel D J Castillejos-López; Ramcés Falfán-Valencia Journal: Diagnostics (Basel) Date: 2020-04-30
Authors: Barbara Dymek; Piotr Sklepkiewicz; Michal Mlacki; Nazan Cemre Güner; Patrycja Nejman-Gryz; Katarzyna Drzewicka; Natalia Przysucha; Aleksandra Rymaszewska; Magdalena Paplinska-Goryca; Agnieszka Zagozdzon; Małgorzata Proboszcz; Łukasz Krzemiński; Jan H von der Thüsen; Katarzyna Górska; Karolina Dzwonek; Zbigniew Zasłona; Pawel Dobrzanski; Rafał Krenke Journal: J Inflamm Res Date: 2022-09-29