Xiwen Wang1, Hang Chi1, Qianxue Li1, Wenliang Li2,3, Jiakuan Li1, Bo Li1, Weicun Gao1, Da Zhang1, Yu Sun1, Le Yi1, Han Qu1, Yutian Wang1, Zhiping Li4, Zhiping Xia5. 1. Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China. 2. Jilin Medical University, Jilin, 132013, China. 3. Key Laboratory of Preparation and Application of Environmental Friendly Materials, Ministry of Education, Jilin Normal University, Changchun, 130103, China. 4. Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China. 14758634@qq.com. 5. Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Military Veterinary Institute, AMMS, Changchun, 130122, China. ammszipxia@163.com.
Abstract
PURPOSE: The present study aims to develop five Gram-negative bacteria expressing bacterial luciferase for use to evaluate the influence of different antibiotics on bacterial bioluminescence. PROCEDURES: The pBBR-lux plasmid was introduced into five Gram-negative bacteria; the bioluminescent signals and colony-forming unit (CFU)/ml of all the bioluminescent strains were monitored with six antibiotics at various concentrations. RESULTS: Dose-dependent bioluminescence signals can be used for rapid bacterial antibiotic susceptibility test (AST). All five bioluminescent bacterial strains have similar bioluminescence and CFU enhancement at sub-minimum inhibitory concentration (MIC) of six different antibiotics. CONCLUSION: The bioluminescent signals and CFU enhancement at sub-MIC antibiotic concentrations should be of value in the research of new antibiotic drugs and bioluminescent imaging.
PURPOSE: The present study aims to develop five Gram-negative bacteria expressing bacterial luciferase for use to evaluate the influence of different antibiotics on bacterial bioluminescence. PROCEDURES: The pBBR-lux plasmid was introduced into five Gram-negative bacteria; the bioluminescent signals and colony-forming unit (CFU)/ml of all the bioluminescent strains were monitored with six antibiotics at various concentrations. RESULTS: Dose-dependent bioluminescence signals can be used for rapid bacterial antibiotic susceptibility test (AST). All five bioluminescent bacterial strains have similar bioluminescence and CFU enhancement at sub-minimum inhibitory concentration (MIC) of six different antibiotics. CONCLUSION: The bioluminescent signals and CFU enhancement at sub-MIC antibiotic concentrations should be of value in the research of new antibiotic drugs and bioluminescent imaging.
Authors: Anton F Engelsman; Isabel C Saldarriaga-Fernandez; M Reza Nejadnik; Gooitzen M van Dam; Kevin P Francis; Rutger J Ploeg; Henk J Busscher; Henny C van der Mei Journal: Biofouling Date: 2010-10 Impact factor: 3.209
Authors: Zhongyi Wang; Hang Chi; Xiwen Wang; Wenliang Li; Zhiping Li; Jiaming Li; Yingying Fu; Bing Lu; Zhiping Xia; Jun Qian; Linna Liu Journal: J Int Med Res Date: 2018-06-07 Impact factor: 1.671