Hong Zeng1, Xiuli Fan1, Nenghui Liu2. 1. Reproductive Medicine Center, Xiangya Hospital, Central South University, 87 XiangYa Road, Changsha, Hunan, 410008, People's Republic of China. 2. Reproductive Medicine Center, Xiangya Hospital, Central South University, 87 XiangYa Road, Changsha, Hunan, 410008, People's Republic of China. dr_liunenghui@126.com.
Abstract
PURPOSE: To detect the expression level of imprinted gene H19 in the endometrium of patients with repeated implantation failure (RIF) who were undergoing in vitro fertilization and embryo transfer (IVF-ET) or frozen embryo transfer (FET). METHODS: Patients who underwent IVF/FET in Reproductive Medicine Center of Xiangya Hospital during April 1, 2015 to October 31, 2015 were chosen. The endometrium was collected from patients with repeated implantation failure during mid-luteal phase. Real-time quantitative reverse transcription PCR (qRT-PCR) and western blot were used to determine the expression of H19 lncRNA and integrin β3 protein. Data analysis was performed using SPSS 19.0. RESULTS: When compared with the control group, decreased expression of H19 was detected in the RIF group (P = 0.04). The expression of integrin β3 protein was also decreased in the RIF group compared to that in the control group (P = 0.04). The expression of H19 lncRNA was positively correlated with the expression of integrin β3 protein (R = 0.477, P = 0.018). CONCLUSIONS: The expression of H19 lncRNA and integrin β3 protein were down-regulated in the RIF patients.
PURPOSE: To detect the expression level of imprinted gene H19 in the endometrium of patients with repeated implantation failure (RIF) who were undergoing in vitro fertilization and embryo transfer (IVF-ET) or frozen embryo transfer (FET). METHODS:Patients who underwent IVF/FET in Reproductive Medicine Center of Xiangya Hospital during April 1, 2015 to October 31, 2015 were chosen. The endometrium was collected from patients with repeated implantation failure during mid-luteal phase. Real-time quantitative reverse transcription PCR (qRT-PCR) and western blot were used to determine the expression of H19 lncRNA and integrin β3 protein. Data analysis was performed using SPSS 19.0. RESULTS: When compared with the control group, decreased expression of H19 was detected in the RIF group (P = 0.04). The expression of integrin β3 protein was also decreased in the RIF group compared to that in the control group (P = 0.04). The expression of H19 lncRNA was positively correlated with the expression of integrin β3 protein (R = 0.477, P = 0.018). CONCLUSIONS: The expression of H19 lncRNA and integrin β3 protein were down-regulated in the RIF patients.
Entities:
Keywords:
H19; In vitro fertilization and embryo transfer; Long non-coding RNA; Repeated implantation failure