Literature DB >> 2879077

Rapid method for micro-analysis of endogenous amino acid neurotransmitters in brain perfusates in the rat by isocratic HPLC-EC.

J M Peinado, K T McManus, R D Myers.   

Abstract

A simplified method is described for the isocratic analysis of endogenous amino acid neurotransmitters contained in brain perfusates by high performance liquid chromatography (HPLC) with electrochemical detection (EC). Pre-column o-phthalaldehyde (OPA) tert-butylthiol derivatives of the amino acids were injected into a C18 3 microns column. After linear concentration curves for standard solutions were obtained, the content of 6 amino acid neurotransmitters was analyzed in push-pull perfusates obtained from the hypothalamus and cerebral cortex of the unrestrained rat. Each analysis which included the simultaneous quantification of aspartic acid, glutamic acid, glutamine, glycine, taurine and gamma-aminobutyric acid (GABA), was completed in less than 15 min. The sensitivity of the assay ranged from 1.0 to 5.0 pmol of each amino acid contained within a 20 microliters aliquot of each perfusion sample.

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Year:  1986        PMID： 2879077     DOI： 10.1016/0165-0270(86)90013-0

Source DB:  PubMed          Journal:  J Neurosci Methods        ISSN： 0165-0270            Impact factor:   2.390


  6 in total

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2.  Strain dependent gene expression and neurochemical levels in the brain of zebrafish: focus on a few alcohol related targets.

Authors:  Y Pan; D Chatterjee; R Gerlai
Journal:  Physiol Behav       Date:  2012-02-01

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Authors:  P C Braga; M Dal Sasso; S Maci; G Bondiolotti; E Fonti; S Reggio
Journal:  Antimicrob Agents Chemother       Date:  1996-10       Impact factor: 5.191

4.  Hemidecortication selectively alters release of glutamate in perfusates collected from cerebral cortex of unrestrained rats.

Authors:  J M Peinado; M C Iribar; R D Myers
Journal:  Neurochem Res       Date:  1987-07       Impact factor: 3.996

5.  Increased intestinal protein synthesis during sepsis and following the administration of tumour necrosis factor alpha or interleukin-1 alpha.

Authors:  D von Allmen; P O Hasselgren; T Higashiguchi; J Frederick; O Zamir; J E Fischer
Journal:  Biochem J       Date:  1992-09-01       Impact factor: 3.857

6.  A rapid assay for neurotransmitter amino acids, aspartate, glutamate, glycine, taurine and gamma-aminobutyric acid in the brain by high-performance liquid chromatography with electrochemical detection.

Authors:  S Murai; H Saito; E Abe; Y Masuda; T Itoh
Journal:  J Neural Transm Gen Sect       Date:  1992
  6 in total

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