Literature DB >> 2878952

Differential immunolabeling for electron microscopy of diverse peptidergic neurons.

M Maegawa, S Hisano, Y Tsuruo, S Katoh, J Nakanishi, M Chikamori-Aoyama, S Daikoku.   

Abstract

We describe a simple and reliable method for differential immunolabeling of pre- and post-synaptic signal peptides at the ultrastructural level. Hypothalamic tissues of rats, including the suprachiasmatic nucleus, were cut on a Vibratome. Visualization of the immunolabeling of somatostatin (SRIH) and vasoactive intestinal polypeptide (VIP) was performed with avidin-biotin-peroxidase-diaminobenzidine (DAB). The end product of the DAB to VIP was further silver-intensified in a physical processing using silver nitrate, and the silver grains were finally substituted for gold. DAB-labeled SRIH fibers synapse on gold-labeled VIP perikarya and dendrites in the suprachiasmatic nucleus.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 2878952     DOI： 10.1177/35.2.2878952

Source DB:  PubMed          Journal:  J Histochem Cytochem        ISSN： 0022-1554            Impact factor:   2.479


  5 in total

1.  Axons containing neuropeptide Y innervate arginine vasopressin-containing neurons in the rat paraventricular nucleus. Dual electron microscopic immunolabeling.

Authors:  Y Kagotani; Y Tsuruo; S Hisano; S Daikoku; K Chihara
Journal:  Histochemistry       Date:  1989

2.  Mutant vasopressin precursor in the endoplasmic reticulum of the Brattleboro rat. Ultrastructural evidence from individual "vasopressin" cells localized with the light microscope by use of a new gold/silver method for immunostain enhancement.

Authors:  S E Guldenaar; B T Pickering
Journal:  Cell Tissue Res       Date:  1988-09       Impact factor: 5.249

3.  Immunohistochemical evidence of serotoninergic regulation of vasoactive intestinal polypeptide (VIP) in the rat suprachiasmatic nucleus.

Authors:  S Hisano; M Chikamori-Aoyama; S Katoh; M Maegawa; S Daikoku
Journal:  Histochemistry       Date:  1987

4.  Synaptic regulation of paraventricular arginine vasopressin-containing neurons by neuropeptide Y-containing monoaminergic neurons in rats. Electron-microscopic triple labeling.

Authors:  Y Kagotani; Y Tsuruo; S Hisano; S Daikoku; K Chihara
Journal:  Cell Tissue Res       Date:  1989-08       Impact factor: 5.249

5.  Ultrastructural localization of immunolabeled substance P and methionine-enkephalin-octapeptide in the surface layer of the dorsal horn of rat spinal cord.

Authors:  S Katoh; S Hisano; S Daikoku
Journal:  Cell Tissue Res       Date:  1988-07       Impact factor: 5.249
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.