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Literature DB >> 2878922

Electrophoretic behavior of the H+-ATPase proteolipid from bovine heart mitochondria.

J Kopecký, J Houstĕk, E Szarska, Z Drahota.

Abstract

The proteolipid subunit of H+-ATPase was labeled by [14C]N,N'-dicyclohexylcarbodiimide in bovine heart mitochondria. The radioactive labeling was followed using various systems of sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). When using discontinuous SDS-PAGE (Laemmli, U.K., 1970, Nature (London) 227, 680-685) a monomeric (Mr 7600 +/- 1500) and a dimeric form (Mr 17,800 +/- 1200) of the proteolipid were detected, while only the monomeric form was found on urea (8 M) containing gels (SDS-PAGE according to Laemmli; or Swank, R. T., and Munkers, K. D., 1971, Anal. Biochem. 39, 462-477). When using SDS-PAGE with Na-Pi buffer (Weber, K., and Osborn, M., 1969, J. Biol. Chem. 244, 4406-4442), only a dimeric form of the proteolipid (Mr 15,000 +/- 1000) was detected. Experimental data indicate that the different patterns of proteolipid separation are related to the presence of the two distinct proteolipid conformations in the SDS solution.

Entities: Chemical Species

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Year: 1986 PMID： 2878922 DOI： 10.1007/bf00743147

Source DB: PubMed Journal: J Bioenerg Biomembr ISSN： 0145-479X Impact factor: 2.945

35 in total

Review 1. Membrane adenosine triphosphatases of prokaryotic cells.

Authors: J A Downie; F Gibson; G B Cox
Journal: Annu Rev Biochem Date: 1979 Impact factor: 23.643

2. Composition of complex V of the mitochondrial oxidative phosphorylation system.

Authors: Y M Galante; S Y Wong; Y Hatefi
Journal: J Biol Chem Date: 1979-12-25 Impact factor: 5.157

3. The dicyclohexylcarbodiimide-binding protein of rat liver mitochondria as a product of the mitochondrial protein synthesis.

Authors: A C Dianoux; M Bof; P V Vignais
Journal: Eur J Biochem Date: 1978-07-17

4. Respiration-driven proton transport in submitochondrial particles.

Authors: P C Hinkle; L L Horstman
Journal: J Biol Chem Date: 1971-10-10 Impact factor: 5.157

5. Identification of the dicyclohexylcarbodiimide-binding protein in the oligomycin-sensitive adenosine triphosphatase from bovine heart mitochondria.

Authors: F S Stekhovan; R F Waitkus; H T Van Moerkerk
Journal: Biochemistry Date: 1972-03-28 Impact factor: 3.162

1. Chemiosmotic energy conversion of the archaebacterial thermoacidophile Sulfolobus acidocaldarius: oxidative phosphorylation and the presence of an F0-related N,N'-dicyclohexylcarbodiimide-binding proteolipid.

Authors: M Lübben; G Schäfer
Journal: J Bacteriol Date: 1989-11 Impact factor: 3.490

1 in total

Electrophoretic behavior of the H+-ATPase proteolipid from bovine heart mitochondria.

Review 1. Membrane adenosine triphosphatases of prokaryotic cells.

2. Composition of complex V of the mitochondrial oxidative phosphorylation system.

3. The dicyclohexylcarbodiimide-binding protein of rat liver mitochondria as a product of the mitochondrial protein synthesis.

4. Respiration-driven proton transport in submitochondrial particles.

5. Identification of the dicyclohexylcarbodiimide-binding protein in the oligomycin-sensitive adenosine triphosphatase from bovine heart mitochondria.

6. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

7. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Review 8. Structure and function of the membrane-integral components of the mitochondrial H+-ATPase.

9. Folding of the mitochondrial proton adenosinetriphosphatase proteolipid channel in phospholipid vesicles.

10. Differentiation of dicyclohexylcarbodiimide reactive sites of the ATPase complex bovine heart mitochondria.

1. Chemiosmotic energy conversion of the archaebacterial thermoacidophile Sulfolobus acidocaldarius: oxidative phosphorylation and the presence of an F0-related N,N'-dicyclohexylcarbodiimide-binding proteolipid.