Literature DB >> 2878337

A rapid and sensitive assay for tyrosine-3-monooxygenase based upon the release of 3H2O and adsorption of [3H]-tyrosine by charcoal.

J F Reinhard, G K Smith, C A Nichol.   

Abstract

A rapid, simple and sensitive assay has been developed for tyrosine-3-monooxygenase, the enzyme catalyzing the rate-limiting step in catecholamine biosynthesis. The assay is based upon the release of 3H2O from 3H-[3,5]-L-tyrosine with adsorption of the isotopic substrate (and its metabolites) by an aqueous slurry of activated charcoal. This method routinely yields low blank values and is simpler than the procedure requiring the use of cation exchange columns to separate the isotopic substrate from the 3H2O formed during the hydroxylation reaction.

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Year:  1986        PMID： 2878337     DOI： 10.1016/0024-3205(86)90395-4

Source DB:  PubMed          Journal:  Life Sci        ISSN： 0024-3205            Impact factor:   5.037


  30 in total

1.  TPH2 in the ventral tegmental area of the male rat brain.

Authors:  Nurgul Carkaci-Salli; Ugur Salli; Kara L Kuntz-Melcavage; Michael M Pennock; Hande Ozgen; Izel Tekin; Willard M Freeman; Kent E Vrana
Journal:  Brain Res Bull       Date:  2011-01-25       Impact factor: 4.077

2.  Double transduction with GTP cyclohydrolase I and tyrosine hydroxylase is necessary for spontaneous synthesis of L-DOPA by primary fibroblasts.

Authors:  C Bencsics; S R Wachtel; S Milstien; K Hatakeyama; J B Becker; U J Kang
Journal:  J Neurosci       Date:  1996-07-15       Impact factor: 6.167

3.  Short-term effects of endothelins on tyrosine hydroxylase activity and expression in the olfactory bulb of normotensive rats.

Authors:  Sabrina L Nabhen; Guadalupe Perfume; María A Battistone; Andrés Rossi; Tamara Abramoff; Liliana G Bianciotti; Marcelo S Vatta
Journal:  Neurochem Res       Date:  2008-10-11       Impact factor: 3.996

4.  Pituitary adenylate cyclase-activating polypeptide is a sympathoadrenal neurotransmitter involved in catecholamine regulation and glucohomeostasis.

Authors:  Carol Hamelink; Olga Tjurmina; Ruslan Damadzic; W Scott Young; Eberhard Weihe; Hyeon-Woo Lee; Lee E Eiden
Journal:  Proc Natl Acad Sci U S A       Date:  2001-12-26       Impact factor: 11.205

5.  Evidence for a functionally important histidine residue in human tyrosine hydroxylase.

Authors:  A Martínez
Journal:  Amino Acids       Date:  1995-09       Impact factor: 3.520

6.  Gender-specific regulation of tyrosine hydroxylase in thymocyte differentiation antigen-1 knockout mice.

Authors:  N Carkaci-Salli; S Battula; X Wang; J R Connor; K E Vrana
Journal:  J Neurosci Res       Date:  2012-03-19       Impact factor: 4.164

7.  Chlamydia pneumoniae encodes a functional aromatic amino acid hydroxylase.

Authors:  Stephanie Abromaitis; P Scott Hefty; Richard S Stephens
Journal:  FEMS Immunol Med Microbiol       Date:  2009-01-09

8.  A biochemical and functional protein complex involving dopamine synthesis and transport into synaptic vesicles.

Authors:  Etienne A Cartier; Leonardo A Parra; Tracy B Baust; Marisol Quiroz; Gloria Salazar; Victor Faundez; Loreto Egaña; Gonzalo E Torres
Journal:  J Biol Chem       Date:  2009-11-10       Impact factor: 5.157

9.  The low affinity dopamine binding site on tyrosine hydroxylase: the role of the N-terminus and in situ regulation of enzyme activity.

Authors:  Sarah L Gordon; Julianne K Webb; Jacqueline Shehadeh; Peter R Dunkley; Phillip W Dickson
Journal:  Neurochem Res       Date:  2009-05-16       Impact factor: 3.996

10.  Tyrosine hydroxylase activity and extrinsic fluorescence changes produced by polyanions.

Authors:  L G Gahn; R Roskoski
Journal:  Biochem J       Date:  1993-10-01       Impact factor: 3.857
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