| Literature DB >> 28781084 |
Adriana Santos Soprano1, Priscila Oliveira de Giuseppe2, Hugo Massayoshi Shimo1, Tatiani Brenelli Lima3, Fernanda Aparecida Heleno Batista1, Germanna Lima Righetto1, José Geraldo de Carvalho Pereira1, Daniela Campos Granato1, Andrey Fabricio Ziem Nascimento4, Fabio Cesar Gozzo5, Paulo Sérgio Lopes de Oliveira1, Ana Carolina Migliorini Figueira1, Juliana Helena Costa Smetana1, Adriana Franco Paes Leme1, Mario Tyago Murakami2, Celso Eduardo Benedetti6.
Abstract
MAF1 is the main RNA polymerase (Pol) III repressor that controls cell growth in eukaryotes. The Citrus ortholog, CsMAF1, was shown to restrict cell growth in citrus canker disease but its role in plant development and disease is still unclear. We solved the crystal structure of the globular core of CsMAF1, which reveals additional structural elements compared with the previously available structure of hMAF1, and explored the dynamics of its flexible regions not present in the structure. CsMAF1 accumulated in the nucleolus upon leaf excision, and this translocation was inhibited by auxin and by mutation of the PKA phosphorylation site, S45, to aspartate. Additionally, mTOR phosphorylated recombinant CsMAF1 and the mTOR inhibitor AZD8055 blocked canker formation in normal but not CsMAF1-silenced plants. These results indicate that the role of TOR on cell growth induced by Xanthomonas citri depends on CsMAF1 and that auxin controls CsMAF1 interaction with Pol III in citrus.Entities:
Keywords: Citrus sinensis; CsMAF1; MAF1; TOR signaling; auxin signaling; human MAF1; plant cell growth; protein kinase A
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Year: 2017 PMID： 28781084 DOI： 10.1016/j.str.2017.07.004
Source DB: PubMed Journal: Structure ISSN： 0969-2126 Impact factor: 5.006